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AbSurance™ Histone H2A, H2B, H4 Antibody Specificity Array

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货号：16-665

品牌：Millipore 密理博

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重要规格表
Description
Catalogue Number 16-665
Trade Name
  • AbSurance
  • Upstate
Description AbSurance™ Histone H2A, H2B, H4 Antibody Specificity Array
Overview Features and Benefits
  • Simple Western blot-like detection procedure
  • High quality ( 〉95 % purity) peptides analyzed by mass spectrometry
  • Two concentrations of peptides allows detection of both strong and weak cross-reactivity
  • Consistent and uniform spotting of peptides using proprietary process
  • Data collection using either film or standard CCD imaging systems
  • Easy- to-read 8 x 12 macroarray – no special imaging equipment or software required
  • PVDF-FL membrane allows sensitive chemiluminescent or fluorescent detection
  • Positive control IgG spots and membrane landmarks for simple and easy spot identification

The AbSurance™ Histone H2A, H2B, H4 Antibody Specificity Array is comprised of 43 high quality histone peptides ( 〉95 % purity) spotted on Immobilon®-FL PVDF membranes in a 96 well format. The Histone H2A, H2B, H4 Array contains 100 ng and 10 ng quantities of modified and unmodified H2A, H2B, H4 peptides: 13 acetylated, 16 methylated, 6 phosphorylated, and 8 unmodified. The Array is also spotted with 10 ng of normal IgG from rat, mouse, sheep, and rabbit, located at the bottom right corner of each Array, to serve as positive controls and location references. The Array is also cut at the upper left corner in the A1 position as an additional location reference mark. Peptides are evenly distributed on the array to enable quantification of distinct spots, and accurate determination of cross-reactivity. The format of the AbSurance™ Histone H2A, H2B, H4 Antibody Specificity Array enables a Western-blot–like detection approach using standard chemiluminescent reagents followed by image capture with either film or a CCD imaging system.
Materials Required but Not Delivered Primary Antibody
Secondary Antibody
Methanol
TBST Solution (Tris-HCl (ph 7.4); 0.15 M NaCl, and 0.05% Tween® 20)
Blocking Solution (5 % non-fat dried milk in TBST)
Immobilon® Western Chemiluminescent HRP Substrate (Cat. No. WBKLS0100) or RapidStep™ ECL Reagent (Cat. No. 345818-100ML)
Containers for the membrane (e.g. Nunc® dishes, Cat. No. 267060)
Shaker platform
Micropipettes and pipette tips
Film or Imager (e.g. Bio-Rad ChemiDoc™ System)
References
Product Information
Detection method Chemiluminescence Fluorescence
Presentation Immobilon®-FL PVDF membranes
Applications
Application Histone Antibody Specificity Screening, Chromatin Immunoprecipitation
Biological Information
Modifications
  • Acetylation
  • Methylation
  • Phosphorylation
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Store at room temperature up to 6 months
Packaging Information
Material Size 1 Array
Transport Information
Supplemental Information
Specifications

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