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Rb X TSH

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货号：AB976

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Ca, H, R, Sh, Fe IH(P) Rb Serum Polyclonal Antibody
Description
Catalogue Number AB976
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Thyroid Stimulating Hormone Antibody
Alternate Names
  • TSH
References
Product Information
Format Serum
Presentation Liquid rabbit antiserum containing 0.1% sodium azide.
Applications
Application Anti-Thyroid Stimulating Hormone Antibody detects level of Thyroid Stimulating Hormone & has been published & validated for use in IH(P).
Key Applications
  • Immunohistochemistry (Paraffin)
Application Notes TISSUE EVALUATION:

Formalin-fixed and paraffin embedded sections (6 microns thick) of anterior pituitary glands were prepared from a number of species including man, cat, rat, dog, ox and sheep. Cryostat sections (10 microns thick) of the above fixed tissues can also be used in the staining procedures.



IMMUNOPEROXIDASE

STAINING:

1. The sections to be stained are hydrated to phosphated buffered saline (PBS, pH 7.4).

2. The antiserum diluted 1:1,000 (in PBS) is allowed to react with the tissue at 37º C for 30 min.

3. Using PBS, the tissue is washed for 15 min at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 min at 37º C. After each of these steps, the tissue is washed with PBS as before.

4. The final stage of the reaction is treatment of the section with a mixture of 3,3'-diaminbenzidine HC1 (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.



IMMUNO-

FLUORESCENCE

STAINING: 1. Same as Step 1 above.

2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 min at 37º C.

3. After washing off the excess antiserum with PBS, the section is treated fluorescein labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 min at 37º C. The tissue is washed with PBS and after mounting under saline-gylcerol, is viewed with a fluorescence microscope.



COMMENTS: The antiserum described here has been established as giving a positive staining on the thyrotrophs when the above protocol is used. Final working dilutions must be determined by end user. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time, e.g. 24 hours at either 37º C or 4º C.
Biological Information
Immunogen Highly purified TSH.
Host Rabbit
Specificity The antiserum supplied has been evaluated for its ability to give a positive and specific immunostaining of thyrotropin stimulating hormone (TSH)-containing cells using both the immunoperoxidase or immunofluorescence procedures.
Species Reactivity
  • Canine
  • Human
  • Rat
  • Sheep
  • Feline
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TSHB
  • TSH-BETA
  • TSH-beta
  • TSH-B
UniProt Number
UniProt Summary FUNCTION: SwissProt: P01222 # Indispensable for the control of thyroid structure and metabolism.
SIZE: 138 amino acids; 15609 Da
SUBUNIT: Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin.
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P01222 ## Belongs to the glycoprotein hormones subunit beta family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Lyophilized: 20°C for up to 18 months. Reconstituted: -20° C for up to 12 months. Do not thaw and refreeze reconstituted material.
Packaging Information
Material Size 500 µL
Transport Information
Supplemental Information
Specifications

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