Anti-Ryanodine Receptor 2 (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	ICC, IHC, WB	Rb	Serum	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

ICC, IHC, WB

Serum

Polyclonal Antibody

Description
Catalogue Number	AB9080
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Ryanodine Receptor 2 Antibody

Description

Catalogue Number

AB9080

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Ryanodine Receptor 2 Antibody

Product Information
Format	Serum
Control	Western blot = adult mouse cardiac muscle or brain (adult mouse skeletal muscle will be negative). IHC = adult mouse cerebellum Purkinje cells (adult mouse cerebellum granular cells will be negative).
Presentation	Rabbit serum. Liquid.

Product Information

Format

Serum

Control

Western blot = adult mouse cardiac muscle or brain (adult mouse skeletal muscle will be negative).

IHC = adult mouse cerebellum Purkinje cells (adult mouse cerebellum granular cells will be negative).

Presentation

Rabbit serum. Liquid.

Applications
Application	Anti-Ryanodine Receptor 2 Antibody is an antibody against Ryanodine Receptor 2 for use in IC, IH & WB.
Key Applications	Immunocytochemistry Immunohistochemistry Western Blotting
Application Notes	Western blot: 1:1,000 using ECL. The antibody reacts with the 500 kDa Ryanodine Receptor 2 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel. Immunocytochemistry: 1:1,000 Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Optimal working dilutions must be determined by the end user.

Applications

Application

Anti-Ryanodine Receptor 2 Antibody is an antibody against Ryanodine Receptor 2 for use in IC, IH & WB.

Key Applications

Immunocytochemistry
Immunohistochemistry
Western Blotting

Application Notes

Western blot: 1:1,000 using ECL. The antibody reacts with the 500 kDa Ryanodine Receptor 2 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.

Immunocytochemistry: 1:1,000

Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Synthetic peptide from the variant TM region of human Ryanodine Receptor 2.
Host	Rabbit
Specificity	Ryanodine Receptor 2.
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_001035.2
Entrez Gene Summary	This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia.
Gene Symbol	RYR2 hRYR-2 ARVD2 RyR2 ARVC2 RYR-2 VTSIP
UniProt Number	Q92736
UniProt Summary	FUNCTION: SwissProt: Q92736 # Communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T- tubules (By similarity). SIZE: 4967 amino acids; 564498 Da SUBUNIT: Homotetramer (Potential). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable). TISSUE SPECIFICITY: Heart muscle, brain (cerebellum and hippocampus) and placenta. DEVELOPMENTAL STAGE: Expressed in myometrium during pregnancy. DISEASE: SwissProt: Q92736 # Defects in RYR2 are the cause of familial arrhythmogenic right ventricular dysplasia 2 (ARVD2) [MIM:600996]; also known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. & Defects in RYR2 are the cause of an autosomal dominant form of stress-induced polymorphic ventricular tachycardia (VTSIP) [MIM:604772]; also known as catecholaminergic polymorphic ventricular tachycardia (CPVT). VTSIP is a genetic arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death. & Defects in RYR2 are a cause of familial polymorphic ventricular tachycardia (FPVT) [MIM:192605]. FPVT is an autosomal- dominant, inherited disease with a relatively early onset and a mortality rate of approximately 30% by the age of 30 years. Phenotypically, it is characterized by salvoes of bidirectional and polymorphic ventricular tachycardias in response to vigorous exercise, with no structural evidence of myocardial disease. SIMILARITY: SwissProt: Q92736 ## Belongs to the ryanodine receptor family. & Contains 3 B30.2/SPRY domains. & Contains 1 EF-hand domain. & Contains 5 MIR domains. MISCELLANEOUS: The calcium release channel is modulated by calcium ions, magnesium ions, ATP and calmodulin. & The calcium release channel activity resides in the C-terminal region while the remaining part of the protein constitutes the 'foot' structure spanning the junctional gap between the SR and the T-tubule. It is possible that the foot structure interacts with the cytoplasmic region of the dihydropyridine receptor. & Ryanodine is an alkaloid that binds to the Ca- release channel in junctional SR and modulates its activity.

Biological Information

Immunogen

Synthetic peptide from the variant TM region of human Ryanodine Receptor 2.

Host

Rabbit

Specificity

Ryanodine Receptor 2.

Species Reactivity

Human
Mouse
Rat

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_001035.2

Entrez Gene Summary

This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia.

Gene Symbol

RYR2
hRYR-2
ARVD2
RyR2
ARVC2
RYR-2
VTSIP

UniProt Number

Q92736

UniProt Summary

FUNCTION: SwissProt: Q92736 # Communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T- tubules (By similarity).
SIZE: 4967 amino acids; 564498 Da
SUBUNIT: Homotetramer (Potential).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable).
TISSUE SPECIFICITY: Heart muscle, brain (cerebellum and hippocampus) and placenta.
DEVELOPMENTAL STAGE: Expressed in myometrium during pregnancy.
DISEASE: SwissProt: Q92736 # Defects in RYR2 are the cause of familial arrhythmogenic right ventricular dysplasia 2 (ARVD2) [MIM:600996]; also known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. & Defects in RYR2 are the cause of an autosomal dominant form of stress-induced polymorphic ventricular tachycardia (VTSIP) [MIM:604772]; also known as catecholaminergic polymorphic ventricular tachycardia (CPVT). VTSIP is a genetic arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death. & Defects in RYR2 are a cause of familial polymorphic ventricular tachycardia (FPVT) [MIM:192605]. FPVT is an autosomal- dominant, inherited disease with a relatively early onset and a mortality rate of approximately 30% by the age of 30 years. Phenotypically, it is characterized by salvoes of bidirectional and polymorphic ventricular tachycardias in response to vigorous exercise, with no structural evidence of myocardial disease.
SIMILARITY: SwissProt: Q92736 ## Belongs to the ryanodine receptor family. & Contains 3 B30.2/SPRY domains. & Contains 1 EF-hand domain. & Contains 5 MIR domains.
MISCELLANEOUS: The calcium release channel is modulated by calcium ions, magnesium ions, ATP and calmodulin. & The calcium release channel activity resides in the C-terminal region while the remaining part of the protein constitutes the 'foot' structure spanning the junctional gap between the SR and the T-tubule. It is possible that the foot structure interacts with the cytoplasmic region of the dihydropyridine receptor. & Ryanodine is an alkaloid that binds to the Ca- release channel in junctional SR and modulates its activity.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Ryanodine Receptor 2 (rabbit polyclonal)

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