Anti-p62 (Sequestosome-1), clone 11C9.2 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R, M	WB, FC, ICC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, R, M

WB, FC, ICC

Purified

Monoclonal Antibody

Description
Catalogue Number	MABC32
Description	Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2
Alternate Names	sequestosome 1 EBI3-associated protein of 60 kDa Paget disease of bone 3 phosphotyrosine independent ligand for the Lck SH2 domain p62 oxidative stress induced like EBI3-associated protein p60 Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Ubiquitin-binding protein p62 sequestosome-1
Background Information	p62 is also known as sequestosome-1, and is a 62 kDa ras-GAP associated protein. p62 is thought to bind to src-family tyrosine kinases and may also provide a linker action for activated src-family kinases with their downstream effectors. p62 has an RNA-binding region and may have RNA-binding ability. p62 is associated with activation of transcription factor NF-κB, involved in cell differentiation, immune response and potassium channel regulation. p62 has recently been linked to extrinsic apoptosis and may be a key player in tumorigenesis.

Description

Catalogue Number

MABC32

Description

Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2

Alternate Names

sequestosome 1
EBI3-associated protein of 60 kDa
Paget disease of bone 3
phosphotyrosine independent ligand for the Lck SH2 domain p62
oxidative stress induced like
EBI3-associated protein p60
Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
Ubiquitin-binding protein p62
sequestosome-1

Background Information

p62 is also known as sequestosome-1, and is a 62 kDa ras-GAP associated protein. p62 is thought to bind to src-family tyrosine kinases and may also provide a linker action for activated src-family kinases with their downstream effectors. p62 has an RNA-binding region and may have RNA-binding ability. p62 is associated with activation of transcription factor NF-κB, involved in cell differentiation, immune response and potassium channel regulation. p62 has recently been linked to extrinsic apoptosis and may be a key player in tumorigenesis.

Product Information
Format	Purified
Control	A431 cell lysate
Presentation	Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Product Information

Format

Purified

Control

A431 cell lysate

Presentation

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Applications
Application	Detect p62 (Sequestosome-1) using this Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 validated for use in WB, FC & IC.
Key Applications	Western Blotting Flow Cytometry Immunocytochemistry
Application Notes	Western Blot Analysis: 0.001 µg/mL from a previous lot detected p62 (Sequestosome-1) in 10 µg of PC12 cell lysate. Flow Cytometry Analysis: 1.0 µg from a previous lot detected p62 (Sequestosome-1) in the staining of fixed and permeabilized HeLa cells. Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected p62 (Sequestosome-1) in NIH/3T3, A431, and HeLa cells.

Applications

Application

Detect p62 (Sequestosome-1) using this Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 validated for use in WB, FC & IC.

Key Applications

Western Blotting
Flow Cytometry
Immunocytochemistry

Application Notes

Western Blot Analysis: 0.001 µg/mL from a previous lot detected p62 (Sequestosome-1) in 10 µg of PC12 cell lysate.

Flow Cytometry Analysis: 1.0 µg from a previous lot detected p62 (Sequestosome-1) in the staining of fixed and permeabilized HeLa cells.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected p62 (Sequestosome-1) in NIH/3T3, A431, and HeLa cells.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to human p62 (Sequestosome-1).
Clone	11C9.2
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Isotype	IgMκ
Species Reactivity	Human Rat Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001135770
Entrez Gene Summary	This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq].
Gene Symbol	PDB3 p62B p62 A170 ZIP3 SQSTM1 p60 OSIL ORCA EBIAP P62
Purification Method	Ion Exchange
UniProt Number	Q13501
UniProt Summary	FUNCTION: Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. SUBUNIT STRUCTURE: Homooligomer or heterooligomer; may form homotypic arrays. Interacts directly with PRKCI and PRKCZ Probable. Forms ternary complexes with PRKCZ and KCNAB2 or PRKCZ and GABBR3. Also interacts with KCNAB1, GABRR1, GABRR2 and GABRR3. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6 (By similarity). Interacts with EBI3, LCK, RASA1, PRKCZ, PRKCI, NR2F2, NTRK1, NTRK2, NTRK3, NBR1, MAP2K5, TRIM55 and MAPKAPK5. Interacts with the proteasome subunits PSMD4 and PSMC2. Interacts with K63-polyubiquitinated MAPT/TAU. Interacts with IKBKB through PRKCZ and PRKCI. Interacts with NGFR through TRAF6 and bridges that complex to NTRK1. Forms a complex with MAP2K5 and PRKCZ or PRKCI. Component of a ternary complex with PAWR and PRKCZ. Upon TNF-alpha stimulation, interacts with RIPK1 problably bridging IKBKB to the TNF-R1 complex composed of TNF-R1/TNFRSF1A, TRADD and RIPK1. Forms a complex with JUB/Ajuba, PRKCZ and TRAF6. Interacts with TRAF6 and CYLD. Identified in a complex with TRAF6 and CYLD (By similarity). SUBCELLULAR LOCATION: Cytoplasm. Late endosome. Nucleus. Note: Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma. TISSUE SPECIFICTY: Ubiquitously expressed. INDUCTION: By proteasomal inhibitor PSI and prostaglandin J2 (PGJ2) (at protein level). By phorbol 12-myristate 13-acetate (PMA). DOMAIN: The UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55. The OPR domain mediates homooligomerization and interactions with PRKCZ, PRKCI, MAP2K5 and NBR1. The ZZ-type zinc finger mediates the interaction with RIPK1. PTM: Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN. INVOLVEMENT IN DISEASE: Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years. SEQUENCE SIMILARITIES: Contains 1 OPR domain. Contains 1 UBA domain. Contains 1 ZZ-type zinc finger.
Molecular Weight	~47-60 kDa observed. The calculated molecular weight of this protein is 47 kDa and also has an isoform at 38 kDa. Due to modifications, this protein may be observed up to ~60 kDa in some lysates.

Biological Information

Immunogen

GST-tagged recombinant protein corresponding to human p62 (Sequestosome-1).

Clone

11C9.2

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Isotype

IgMκ

Species Reactivity

Human
Rat
Mouse

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_001135770

Entrez Gene Summary

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq].

Gene Symbol

PDB3
p62B
p62
A170
ZIP3
SQSTM1
p60
OSIL
ORCA
EBIAP
P62

Purification Method

Ion Exchange

UniProt Number

Q13501

UniProt Summary

FUNCTION: Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.

SUBUNIT STRUCTURE: Homooligomer or heterooligomer; may form homotypic arrays. Interacts directly with PRKCI and PRKCZ Probable. Forms ternary complexes with PRKCZ and KCNAB2 or PRKCZ and GABBR3. Also interacts with KCNAB1, GABRR1, GABRR2 and GABRR3. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6 (By similarity). Interacts with EBI3, LCK, RASA1, PRKCZ, PRKCI, NR2F2, NTRK1, NTRK2, NTRK3, NBR1, MAP2K5, TRIM55 and MAPKAPK5. Interacts with the proteasome subunits PSMD4 and PSMC2. Interacts with K63-polyubiquitinated MAPT/TAU. Interacts with IKBKB through PRKCZ and PRKCI. Interacts with NGFR through TRAF6 and bridges that complex to NTRK1. Forms a complex with MAP2K5 and PRKCZ or PRKCI. Component of a ternary complex with PAWR and PRKCZ. Upon TNF-alpha stimulation, interacts with RIPK1 problably bridging IKBKB to the TNF-R1 complex composed of TNF-R1/TNFRSF1A, TRADD and RIPK1. Forms a complex with JUB/Ajuba, PRKCZ and TRAF6. Interacts with TRAF6 and CYLD. Identified in a complex with TRAF6 and CYLD (By similarity).

SUBCELLULAR LOCATION: Cytoplasm. Late endosome. Nucleus. Note: Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma.

TISSUE SPECIFICTY: Ubiquitously expressed.

INDUCTION: By proteasomal inhibitor PSI and prostaglandin J2 (PGJ2) (at protein level). By phorbol 12-myristate 13-acetate (PMA).

DOMAIN: The UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55.

The OPR domain mediates homooligomerization and interactions with PRKCZ, PRKCI, MAP2K5 and NBR1.

The ZZ-type zinc finger mediates the interaction with RIPK1.

PTM: Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN.

INVOLVEMENT IN DISEASE: Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years.

SEQUENCE SIMILARITIES: Contains 1 OPR domain.

Contains 1 UBA domain.

Contains 1 ZZ-type zinc finger.

Molecular Weight

~47-60 kDa observed.
The calculated molecular weight of this protein is 47 kDa and also has an isoform at 38 kDa. Due to modifications, this protein may be observed up to ~60 kDa in some lysates.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in A431 cell lysate. Western Blot Analysis: 0.001 µg/mL of this antibody detected p62 (Sequestosome-1) in 10 µg of A431 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 0.001 µg/mL of this antibody detected p62 (Sequestosome-1) in 10 µg of A431 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-p62 (Sequestosome-1), clone 11C9.2 (mouse monoclonal)

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