Gt X Apolipoprotein E

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Pm	IHC, WB	Gt	Serum	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, Pm

IHC, WB

Serum

Polyclonal Antibody

Description
Catalogue Number	AB947
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Apolipoprotein E Antibody
Alternate Names	ApoE

Description

Catalogue Number

AB947

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Apolipoprotein E Antibody

Alternate Names

ApoE

Product Information
Format	Serum
Control	Liver tissue
Presentation	Delipidated goat antiSerum, 0.2 μm filtered. Liquid in Tris-saline, pH 7.4 with 0.1% sodium azide as preservative.

Product Information

Format

Serum

Control

Liver tissue

Presentation

Delipidated goat antiSerum, 0.2 μm filtered. Liquid in Tris-saline, pH 7.4 with 0.1% sodium azide as preservative.

Applications
Application	Detect Apolipoprotein E using this Anti-Apolipoprotein E Antibody validated for use in IH & WB.
Key Applications	Immunohistochemistry Western Blotting
Application Notes	Western blot (Schwab, 1996): 1:1,000-1:2,000 Immunohistochemistry (Summers, 1998; Wisniewski & Frangione, 1992; Schwab, 1996) Optimal working dilutions must be determined by the end user.

Applications

Application

Detect Apolipoprotein E using this Anti-Apolipoprotein E Antibody validated for use in IH & WB.

Key Applications

Immunohistochemistry
Western Blotting

Application Notes

Western blot (Schwab, 1996): 1:1,000-1:2,000

Immunohistochemistry (Summers, 1998; Wisniewski & Frangione, 1992; Schwab, 1996)

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Recombinant human apolipoprotein E.
Host	Goat
Specificity	Monospecific for Apolipoprotein E by immunoelectrophoresis when tested against pooled human plasma and twice concentrated pooled human serum. Reacts with Apo E isoforms E2, E3 and E4 by Western blot.
Species Reactivity	Human Primate
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_000041.2
Entrez Gene Summary	Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
Gene Symbol	APOE Apo-E apoprotein MGC1571 AD2
Purification Method	Unpurified
UniProt Number	P02649
UniProt Summary	FUNCTION: SwissProt: P02649 # Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. SIZE: 317 amino acids; 36154 Da SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle. PTM: Synthesized with the sialic acid attached by O-glycosidic linkage and is subsequently desialylated in plasma. & Glycated in plasma VLDL of normal subjects, and of hyperglycemic diabetic patients at a higher level (2-3 fold). DISEASE: SwissProt: P02649 # Defects in APOE are a cause of hyperlipoproteinemia type III [MIM:107741]; also known as familial dysbetalipoproteinemia. Individuals with hyperlipoproteinemia type III, are clinically characterized by xanthomas, yellowish lipid deposits in the palmar crease, or less specific on tendons and on elbows. The disorder rarely manifests before the third decade in men. In women, it is usually expressed only after the menopause. The vast majority of the patients are homozygous for APOE2 alleles. More severe cases of hyperlipoproteinemia type III have also been observed in individuals heterozygous for rare APOE variants. The influence of APOE on lipid levels is often suggested to have major implications for the risk of coronary artery disease (CAD). Individuals carrying the common APOE4 variant are at higher risk of CAD. & The APOE4 allele is associated with late onset Alzheimer disease 2 (AD2) [MIM:104310]. The APOE4 allele is genetically associated with the common late onset familial and sporadic forms of Alzheimer disease (AD). Risk for AD increased from 20% to 90% and mean age at onset decreased from 84 to 68 years with increasing number of APOE4 alleles in 42 families with late onset AD. Thus APOE4 gene dose is a major risk factor for late onset AD and, in these families, homozygosity for APOE4 was virtually sufficient to cause AD by age 80. The mechanism by which APOE4 participates in pathogenesis is not known. & Defects in APOE are a cause of sea-blue histiocyte disease [MIM:269600]; also called sea-blue histiocytosis. This disorder is characterized by splenomegaly, mild thrombocytopenia and, in the bone marrow, numerous histiocytes containing cytoplasmic granules which stain bright blue with the usual hematologic stains. The syndrome is the consequence of an inherited metabolic defect analogous to Gaucher disease and other sphingolipidoses. SIMILARITY: SwissProt: P02649 ## Belongs to the apolipoprotein A1/A4/E family.
Molecular Weight	34 kDa

Biological Information

Immunogen

Recombinant human apolipoprotein E.

Host

Goat

Specificity

Monospecific for Apolipoprotein E by immunoelectrophoresis when tested against pooled human plasma and twice concentrated pooled human serum. Reacts with Apo E isoforms E2, E3 and E4 by Western blot.

Species Reactivity

Human
Primate

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_000041.2

Entrez Gene Summary

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.

Gene Symbol

APOE
Apo-E
apoprotein
MGC1571
AD2

Purification Method

Unpurified

UniProt Number

P02649

UniProt Summary

FUNCTION: SwissProt: P02649 # Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
SIZE: 317 amino acids; 36154 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.
PTM: Synthesized with the sialic acid attached by O-glycosidic linkage and is subsequently desialylated in plasma. & Glycated in plasma VLDL of normal subjects, and of hyperglycemic diabetic patients at a higher level (2-3 fold).
DISEASE: SwissProt: P02649 # Defects in APOE are a cause of hyperlipoproteinemia type III [MIM:107741]; also known as familial dysbetalipoproteinemia. Individuals with hyperlipoproteinemia type III, are clinically characterized by xanthomas, yellowish lipid deposits in the palmar crease, or less specific on tendons and on elbows. The disorder rarely manifests before the third decade in men. In women, it is usually expressed only after the menopause. The vast majority of the patients are homozygous for APOE*2 alleles. More severe cases of hyperlipoproteinemia type III have also been observed in individuals heterozygous for rare APOE variants. The influence of APOE on lipid levels is often suggested to have major implications for the risk of coronary artery disease (CAD). Individuals carrying the common APOE*4 variant are at higher risk of CAD. & The APOE*4 allele is associated with late onset Alzheimer disease 2 (AD2) [MIM:104310]. The APOE*4 allele is genetically associated with the common late onset familial and sporadic forms of Alzheimer disease (AD). Risk for AD increased from 20% to 90% and mean age at onset decreased from 84 to 68 years with increasing number of APOE*4 alleles in 42 families with late onset AD. Thus APOE*4 gene dose is a major risk factor for late onset AD and, in these families, homozygosity for APOE*4 was virtually sufficient to cause AD by age 80. The mechanism by which APOE*4 participates in pathogenesis is not known. & Defects in APOE are a cause of sea-blue histiocyte disease [MIM:269600]; also called sea-blue histiocytosis. This disorder is characterized by splenomegaly, mild thrombocytopenia and, in the bone marrow, numerous histiocytes containing cytoplasmic granules which stain bright blue with the usual hematologic stains. The syndrome is the consequence of an inherited metabolic defect analogous to Gaucher disease and other sphingolipidoses.
SIMILARITY: SwissProt: P02649 ## Belongs to the apolipoprotein A1/A4/E family.

Molecular Weight

34 kDa

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Storage and Shipping Information

Storage Conditions

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Packaging Information
Material Size	1 mL

Packaging Information

Material Size

1 mL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

浏览了该商品的用户最终购买

浏览了该商品的用户还浏览了

特卖

重要规格表

我要咨询