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Ms X Hu Cytokeratin 19

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货号：CBL198

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, ICC, IH(P) M Purified Monoclonal Antibody
Description
Catalogue Number CBL198
Replaces CBL178
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Cytokeratin 19 Antibody, clone BA 17
References
Product Information
Format Purified
Presentation The monoclonal is presented at a concentration of 0.1mg/1ml in phosphate buffered saline containing 10mM sodium azide and 1% bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Applications
Application Detect Cytokeratin 19 using this Anti-Cytokeratin 19 Antibody, clone BA 17 validated for use in WB, IC, IH(P).
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
Application Notes Immunocytochemistry

Western blotting

Suitable for staining frozen and paraffin wax embedded tissue sections after proteolytic enzyme digestion

Cytokeratin 19 positive detection distinguishes malignant from benign breast tumours

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Human reduction mammoplasty organoids
Clone BA 17
Host Mouse
Specificity The antibody is specific to human cytokeratin 19 and is reactive with cells of epithelial origin. Cytokeratin 19 is the smallest human cytokeratin (40 kDa) and is found in many simple and invariably in some non-keratinising stratified squamous epithelia. All adenocarcinomas tested have been positive for cytokeratin 19.

FUSION PARTNER: Px63 Ag 8.653 myeloma cell line
Isotype IgG1
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.
Gene Symbol
  • KRT19
  • Keratin-19
  • K19
  • CK19
  • MGC15366
  • K1CS
  • Cytokeratin-19
  • CK-19
UniProt Number
UniProt Summary FUNCTION: SwissProt: P08727 # Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
SIZE: 400 amino acids; 44092 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins. Interacts with PNN and the actin-binding domain of DMD. Interacts with HCV core protein.
TISSUE SPECIFICITY: Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.DEVELOPMENTAL STAGE: Present in hair follicles at all stages of development.
DOMAIN: SwissProt: P08727 This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
SIMILARITY: Belongs to the intermediate filament family.
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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