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Anti-Epithelial Specific Antigen, clone VU-1D9 (mouse monoclonal)

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货号：CBL251

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, ICC, IHC M Purified Monoclonal Antibody
Description
Catalogue Number CBL251
Description Anti-Epithelial Specific Antigen Antibody, clone VU-1D9
Alternate Names
  • Epithelial cell adhesion molecule
  • Ep-CAM
  • Adenocarcinoma-associated antigen
  • Cell surface glycoprotein Trop-1
  • Epithelial cell surface antigen
  • Epithelial glycoprotein
  • EGP
  • Epithelial glycoprotein 314
  • EGP314
  • hEGP314
  • KS 1/4 antigen
  • KSA
  • Major gastrointestinal tumor-associated protein GA733-2
  • Tumor-associated calcium signal transducer 1
  • CD326
Background Information Epithelial specific antigen, also known as epithelial cell adhesion molecule (Ep-CAM) and epithelial glycoprotein (EGP), may act as an interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Epithelial specific antigen plays a role in embryonic stem cells proliferation and differentiation, and up-regulates the expression of FABP5, MYC, and cyclins A and E. Epithelial specific antigen is highly and selectively expressed by undifferentiated embryonic stem cells (ESCs). Protein levels rapidly diminish as soon as ESCs differentiate. Also expressed in most epithelial cell membranes and on the surface of adenocarcinoma.
References
Product Information
Format Purified
Control
  • MCF7 cell lyasate
Presentation Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.
Applications
Application Anti-Epithelial Specific Antigen Antibody, clone VU-1D9 is a highly specific mouse monoclonal antibody, that targets Epithelial Cell Marker & has been tested in western blotting, ICC & IHC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application Notes Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Epithelial Specific Antigen in A431 cells.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Epithelial Specific Antigen in human prostate adenocarcinoma cells.
Biological Information
Immunogen NCI-H69 whole cells
Clone VU-1D9
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG1κ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • EPCAM
  • GA733-2
  • M1S2
  • M4S1
  • MIC18
  • TACSTD1
  • TROP1
Purification Method Protein G Purified
UniProt Number
Molecular Weight ~40 kDa observed. The calculated molecular weight of this protein is 35 kDa; however, this protein will run higher due to glycosylation.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in MCF7 cell lyate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Epithelial Specific Antigen in 10 µg of MCF7 cell lyate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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