Rb X MLH1

Species Reactivity	Key Applications	Host	Format	Antibody Type
B, Ca, H, R, Sh, Fe	IH(P)	Rb	Serum	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

B, Ca, H, R, Sh, Fe

IH(P)

Serum

Polyclonal Antibody

Description
Catalogue Number	AB902
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Adrenocorticotropic Hormone Antibody
Alternate Names	ACTH
Background Information	ANTIGEN: Synthetic ACTH (1-24) bound to keyhole limpet hemocyanin (KLH) with carbodiimide.

Description

Catalogue Number

AB902

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Adrenocorticotropic Hormone Antibody

Alternate Names

ACTH

Background Information

ANTIGEN:

Synthetic ACTH (1-24) bound to keyhole limpet hemocyanin (KLH) with carbodiimide.

Product Information
Format	Serum
Presentation	Liquid. Contains 0.1% sodium azide.

Product Information

Format

Serum

Presentation

Liquid. Contains 0.1% sodium azide.

Applications
Application	Anti-Adrenocorticotropic Hormone Antibody detects level of Adrenocorticotropic Hormone & has been published & validated for use in IH(P).
Key Applications	Immunohistochemistry (Paraffin)
Application Notes	TISSUE EVALUATION: Formalin-fixed and paraffin-embedded sections (6 microns thick) of anterior pituitary glands were prepared from a number of species including man, dog, rat, cat, ox and sheep. Cryostat sections of the above tissued can also be used in the staining procedures. IMMUNOPEROXIDASE STAINING: 1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4). 2. The antiserum diluted 1:1,000 - 1:2,000 (in PBS) is allowed to react with the tissue at 37° C for 30 minutes. 3.Using PBS, the tissue is washed for 15 min at room temperature and treated sequentially with sheep anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 37°C. After each of these two steps excess reagent is washed from the section as described above. 4. The final step in the staining involves treating the section with a mixture of 3,3'- diaminbenzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted. IMMUNOFLUORESCENCE STAINING: 1. Same as Step 1 above. 2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37°C. 3. After washing off the excess reagents with PBS, the section is treated with fluoresceinlabeled sheep anti-rabbit gamma globulin (diluted 1:10 in PBS) for 30 min at 37° C. The tissue is washed with PBS and after mounting under glycerine-saline is viewed with a fluorescence microscope. COMMENTS: The antiserum dilutions described have been established as giving a positive staining on the corticotrophs using the above protocol. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time e.g. 24 hr.

Applications

Application

Anti-Adrenocorticotropic Hormone Antibody detects level of Adrenocorticotropic Hormone & has been published & validated for use in IH(P).

Key Applications

Immunohistochemistry (Paraffin)

Application Notes

TISSUE EVALUATION:

Formalin-fixed and paraffin-embedded sections (6 microns thick) of anterior pituitary glands were prepared from a number of species including man, dog, rat, cat, ox and sheep. Cryostat sections of the above tissued can also be used in the staining procedures.

IMMUNOPEROXIDASE

STAINING:

1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4).

2. The antiserum diluted 1:1,000 - 1:2,000 (in PBS) is allowed to react with the tissue at 37° C for 30 minutes.

3.Using PBS, the tissue is washed for 15 min at room temperature and treated sequentially with sheep anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 37°C. After each of these two steps excess reagent is washed from the section as described above.

4. The final step in the staining involves treating the section with a mixture of 3,3'- diaminbenzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.

IMMUNOFLUORESCENCE

STAINING:

1. Same as Step 1 above.

2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37°C.

3. After washing off the excess reagents with PBS, the section is treated with fluoresceinlabeled sheep anti-rabbit gamma globulin (diluted 1:10 in PBS) for 30 min at 37° C. The tissue is washed with PBS and after mounting under glycerine-saline is viewed with a fluorescence microscope.

COMMENTS:

The antiserum dilutions described have been established as giving a positive staining on the corticotrophs using the above protocol. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time e.g. 24 hr.

Biological Information
Host	Rabbit
Specificity	The antiserum provided has been evaluated for its ability to give a specific and positive immunostaining of ACTH-containing cells using both the immunoperoxidase and immunofluoresence procedures.
Species Reactivity	Bovine Canine Human Rat Sheep Feline
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_000939.2 NM_001035256.1
Entrez Gene Summary	This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
Gene Symbol	POMC pro-ACTH-endorphin adrenocorticotropin beta-LPH POC gamma-LPH LPH ACTH corticotropin beta-endorphin beta-MSH alpha-MSH pro-opiomelanocortin proopiomelanocortin Pro-opiomelanocortin corticotropin-lipotrophin NPP MSH met-enkephalin CLIP gamma-MSH
UniProt Number	P01189
UniProt Summary	FUNCTION: SwissProt: P01189 # Beta-endorphin and Met-enkephalin are endogenous opiates. SIZE: 267 amino acids; 29424 Da TISSUE SPECIFICITY: ACTH and MSH are produced by the pituitary gland. PTM: Specific enzymatic cleavages at paired basic residues yield the different active peptides. & O-glycosylated; reducing sugar is probably N- acetylgalactosamine. DISEASE: SwissProt: P01189 # Defects in POMC may be associated with susceptibility to obesity [MIM:601665]. & Defects in POMC are the cause of pro-opiomelanocortinin deficiency [MIM:609734]. Affected individuals present early-onset obesity, adrenal insufficiency and red hair. SIMILARITY: SwissProt: P01189 ## Belongs to the POMC family.

Biological Information

Host

Rabbit

Specificity

The antiserum provided has been evaluated for its ability to give a specific and positive immunostaining of ACTH-containing cells using both the immunoperoxidase and immunofluoresence procedures.

Species Reactivity

Bovine
Canine
Human
Rat
Sheep
Feline

Antibody Type

Polyclonal Antibody

Entrez Gene Number

Entrez Gene Summary

This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.

Gene Symbol

POMC
pro-ACTH-endorphin
adrenocorticotropin
beta-LPH
POC
gamma-LPH
LPH
ACTH
corticotropin
beta-endorphin
beta-MSH
alpha-MSH
pro-opiomelanocortin
proopiomelanocortin
Pro-opiomelanocortin
corticotropin-lipotrophin
NPP
MSH
met-enkephalin
CLIP
gamma-MSH

UniProt Number

P01189

UniProt Summary

FUNCTION: SwissProt: P01189 # Beta-endorphin and Met-enkephalin are endogenous opiates.
SIZE: 267 amino acids; 29424 Da
TISSUE SPECIFICITY: ACTH and MSH are produced by the pituitary gland.
PTM: Specific enzymatic cleavages at paired basic residues yield the different active peptides. & O-glycosylated; reducing sugar is probably N- acetylgalactosamine.
DISEASE: SwissProt: P01189 # Defects in POMC may be associated with susceptibility to obesity [MIM:601665]. & Defects in POMC are the cause of pro-opiomelanocortinin deficiency [MIM:609734]. Affected individuals present early-onset obesity, adrenal insufficiency and red hair.
SIMILARITY: SwissProt: P01189 ## Belongs to the POMC family.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store at -20°C in undiluted aliquots for up to 12 months. Do not thaw and refreeze material.

Storage and Shipping Information

Storage Conditions

Store at -20°C in undiluted aliquots for up to 12 months. Do not thaw and refreeze material.

Packaging Information
Material Size	500 µL

Packaging Information

Material Size

500 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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