Anti-Nectin-1/PVRL1 (CD111), clone R1.302 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	FC, IF	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

FC, IF

Purified

Monoclonal Antibody

Description
Catalogue Number	MABT61
Description	Anti-Nectin-1/PVRL1 (CD111) Antibody, clone R1.302
Alternate Names	poliovirus receptor-related 1 (herpesvirus entry mediator C) nectin-1 poliovirus receptor-related protein 1 poliovirus receptor-like 1 nectin 1 ectodermal dysplasia 4 (Margarita Island type) Herpesvirus entry mediator C Herpes virus entry mediator C Nectin-1 Herpesvirus Ig-like receptor CD111 antigen
Background Information	Nectin-1, also known as poliovirus receptor-related protein 1 (PRR1 or PVRL1) or CD111, is an immunoglobulin-like transmembrane cell adhesion protein that also seems to play a role in neural tube formation, with N-cadherin. Nectins are known for being the receptors for the herpes simplex virus. Nectin-1 is connected with the actin cytoskeleton through afadin inside the cell. It is predominantly found in human placenta although in mice, expression is wide-ranging. Nectin-1 seems to adhere primarily to cells expressing nectin-4 and vice versa.

Description

Catalogue Number

MABT61

Description

Anti-Nectin-1/PVRL1 (CD111) Antibody, clone R1.302

Alternate Names

poliovirus receptor-related 1 (herpesvirus entry mediator C)
nectin-1
poliovirus receptor-related protein 1
poliovirus receptor-like 1
nectin 1
ectodermal dysplasia 4 (Margarita Island type)
Herpesvirus entry mediator C
Herpes virus entry mediator C
Nectin-1
Herpesvirus Ig-like receptor
CD111 antigen

Background Information

Nectin-1, also known as poliovirus receptor-related protein 1 (PRR1 or PVRL1) or CD111, is an immunoglobulin-like transmembrane cell adhesion protein that also seems to play a role in neural tube formation, with N-cadherin. Nectins are known for being the receptors for the herpes simplex virus. Nectin-1 is connected with the actin cytoskeleton through afadin inside the cell. It is predominantly found in human placenta although in mice, expression is wide-ranging. Nectin-1 seems to adhere primarily to cells expressing nectin-4 and vice versa.

Product Information
Format	Purified
Control	Human TF-1 cells
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

Human TF-1 cells

Presentation

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	This Anti-Nectin-1/PVRL1 (CD111) Antibody, clone R1.302 is validated for use in FC, IF for the detection of Nectin-1/PVRL1 (CD111).
Key Applications	Flow Cytometry Immunofluorescence
Application Notes	Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF (Cocchi, F., et al. (1998). Journal of Virology. 72(12):9992–10002).

Applications

Application

This Anti-Nectin-1/PVRL1 (CD111) Antibody, clone R1.302 is validated for use in FC, IF for the detection of Nectin-1/PVRL1 (CD111).

Key Applications

Flow Cytometry
Immunofluorescence

Application Notes

Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF (Cocchi, F., et al. (1998). Journal of Virology. 72(12):9992–10002).

Biological Information
Immunogen	Human CD111 transfected NIH/3T3 cells
Clone	R1.302.12
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_002846
Entrez Gene Summary	This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini. [provided by RefSeq].
Gene Symbol	PVRR PRR1 PVRR1 CLPED1 OFC7 HVEC SK-12 PRR CD111 ED4 PVRL1 HIgR HveC
Purification Method	Protein G Purified
UniProt Number	Q15223
UniProt Summary	FUNCTION: Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4. SUBUNIT STRUCTURE: Can form trans-heterodimers with PVRL3/nectin-3 and with PVRL4/nectin-4. Interacts (via C-terminus) with afadin (via PDZ domain); this interaction recruits PVRL1 to cadherin-based adherens junctions. Interacts with integrin alphaV/beta3. Interacts with herpes simplex virus 1 (HHV-1), herpes simplex virus 2 (HHV-2), and pseudorabies virus (PRV) envelope glycoprotein D; functions as an entry receptor for these viruses. SUBCELLULAR LOCATION: Isoform Alpha: Cell membrane; Single-pass type I membrane protein. Isoform Delta: Cell membrane; Single-pass type I membrane protein. Isoform Gamma: Secreted. INVOLVEMENT IN DISEASE: Defects in PVRL1 are the cause of ectodermal dysplasia Margarita Island type (EDMI) [MIM:225060]; also known as Zlotogora-Ogur syndrome, cleft lip/palate-ectodermal dysplasia syndrome (CLPED1) or ectodermal dysplasia 4. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDMI is an autosomal recessive syndrome characterized by the association of cleft lip/palate, ectodermal dysplasia (sparse short and dry scalp hair, sparse eyebrows and eyelashes), and partial syndactyly of the fingers and/or toes. Two thirds of the patients do not manifest oral cleft but present with abnormal teeth and nails. Defects in PVRL1 are the cause of non-syndromic orofacial cleft type 7 (OFC7) [MIM:225060]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. SEQUENCE SIMILARITIES: Belongs to the nectin family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SEQUENCE CAUTION: The sequence CAA53980.2 differs from that shown. Reason: Erroneous initiation.
Molecular Weight	57 kDa calculated

Biological Information

Immunogen

Human CD111 transfected NIH/3T3 cells

Clone

R1.302.12

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Isotype

IgG1κ

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_002846

Entrez Gene Summary

This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini. [provided by RefSeq].

Gene Symbol

PVRR
PRR1
PVRR1
CLPED1
OFC7
HVEC
SK-12
PRR
CD111
ED4
PVRL1
HIgR
HveC

Purification Method

Protein G Purified

UniProt Number

Q15223

UniProt Summary

FUNCTION: Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4.

SUBUNIT STRUCTURE: Can form trans-heterodimers with PVRL3/nectin-3 and with PVRL4/nectin-4. Interacts (via C-terminus) with afadin (via PDZ domain); this interaction recruits PVRL1 to cadherin-based adherens junctions. Interacts with integrin alphaV/beta3. Interacts with herpes simplex virus 1 (HHV-1), herpes simplex virus 2 (HHV-2), and pseudorabies virus (PRV) envelope glycoprotein D; functions as an entry receptor for these viruses.

SUBCELLULAR LOCATION: Isoform Alpha: Cell membrane; Single-pass type I membrane protein.

Isoform Delta: Cell membrane; Single-pass type I membrane protein.

Isoform Gamma: Secreted.

INVOLVEMENT IN DISEASE: Defects in PVRL1 are the cause of ectodermal dysplasia Margarita Island type (EDMI) [MIM:225060]; also known as Zlotogora-Ogur syndrome, cleft lip/palate-ectodermal dysplasia syndrome (CLPED1) or ectodermal dysplasia 4. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDMI is an autosomal recessive syndrome characterized by the association of cleft lip/palate, ectodermal dysplasia (sparse short and dry scalp hair, sparse eyebrows and eyelashes), and partial syndactyly of the fingers and/or toes. Two thirds of the patients do not manifest oral cleft but present with abnormal teeth and nails.

Defects in PVRL1 are the cause of non-syndromic orofacial cleft type 7 (OFC7) [MIM:225060]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.

SEQUENCE SIMILARITIES: Belongs to the nectin family.

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SEQUENCE CAUTION: The sequence CAA53980.2 differs from that shown. Reason: Erroneous initiation.

Molecular Weight

57 kDa calculated

Product Usage Statements
Quality Assurance	Evaluated by Flow Cytometry in human TF-1 cells. Flow Cytometry Analysis: 0.25 µg of this antibody detected Nectin-1/PVRL1 in human TF-1 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Flow Cytometry in human TF-1 cells.

Flow Cytometry Analysis: 0.25 µg of this antibody detected Nectin-1/PVRL1 in human TF-1 cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	50 µg

Packaging Information

Material Size

50 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Nectin-1/PVRL1 (CD111), clone R1.302 (mouse monoclonal)

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