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Anti-DDX4 (Vasa) (rabbit polyclonal)

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货号：AB4330

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IH(P) Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB4330
Description Anti-DDX4 (Vasa) Antibody
Alternate Names
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
  • probable ATP-dependent RNA helicase DDX4
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4
  • Vasa homolog
  • DEAD box protein 4
Background Information DDX4 (Vasa or MVH) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. DDX4 (Vasa) proteins are mostly localized in testicular germ cells in the cytoplasm of spermatocytes. DDX4 (Vasa) is a high-copy extragenic suppressor gene. DDX4 (Vasa) is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility.
References
Product Information
Format Affinity Purified
Control
  • Mouse testis tissue lysate
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application Anti-DDX4 (Vasa) Antibody detects level of DDX4 (Vasa) & has been published & validated for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application Notes Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected DDX4 (Vasa) in normal human testis tissue.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to the C-terminus of mouse DDX4 (Vasa).
Epitope C-terminus
Host Rabbit
Specificity This antibody recognizes DDX4 (Vasa) at the C-terminus.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • VASA
  • MVH
  • DDX4
Purification Method Affinity Purfied
UniProt Number
UniProt Summary FUNCTION: May play a role in germ cell development.

CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.

SUBUNIT STRUCTURE: Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL. Ref.3 SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › perinuclear region.

TISSUE SPECIFICTY: Testis.

DEVELOPMENTAL STAGE: Expressed in spermatogenic cells from the spermatocyte stage to the round spermatid stage.

SEQUENCE SIMILARITIES: Belongs to the DEAD box helicase family. DDX4/VASA subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.
Molecular Weight ~76 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in mouse testis tissue lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected DDX4 (Vasa) in 10 µg of mouse testis tissue lysate. Western Blot performed without Tween.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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