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Mx X GABA(A) Receptor alpha Chain

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货号：MAB339

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Av, B, H, Po IHC, IP, WB M Purified Monoclonal Antibody
Description
Catalogue Number MAB339
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-GABA A Receptor α1 chain Antibody, NT, clone BD24
References
Product Information
Format Purified
Presentation Liquid. Buffer = 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Applications
Application Anti-GABA A Receptor α1 chain Antibody, N-terminus, clone BD24 is an antibody against GABA A Receptor α1 chain for use in IH, IP & WB.
Key Applications
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application Notes Immunohistochemistry: 10-20 μg/mL * See protocol below.

Western blot: 20 μg/mL

Immunoprecipitation

Optimal working dilutions must be determined by end user.

APPLICATION NOTES FOR MAB339

IMMUNOHISTOCHEMISTRY

1) Fresh tissue (human) should be used (3-6 hours postmortem). The tissue should be immersion fixed in 2% paraformaldehyde/0.1% glutaraldehyde. Prepare 50 mm sections and store in cryoprotective solution at -15°C.

2) Sections should be incubated floating in suitable small vials.

3) Block endogenous peroxidase with 100 mmol/L Tris-HCl, 150 mmol/L NaCl, pH 7.4 (Buffer A) containing 3% H2O2 (v/v) and 10% methanol for 20 min at room temperature.

4) Wash sections in Buffer A. Incubate sections in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v).

5) Wash sections in Buffer A. Incubate sections with MAB339 (diluted 10-20 mg/mL in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v) for 12-36 hours at +4°C

6) Wash sections in Buffer A.

7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).

8) Wash sections in Buffer A.

9) Mount sections on chrome alum-coated slides, dry, dehydrate, and apply coverslips.
Biological Information
Immunogen Purified GABA/benzodiazepine receptor from bovine cortex.
Epitope N-terminus
Clone BD24
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Reacts with the alpha 1-chain of the GABAA receptor in bovine and human but not rat
Isotype IgG1
Species Reactivity
  • Avian
  • Bovine
  • Human
  • Pig
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified.
Gene Symbol
  • GABRA1
  • ECA4
  • GABA
  • EJM
Non-Reactive Species
  • Rat
  • Feline
UniProt Number
UniProt Summary FUNCTION: SwissProt: P14867 # GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
SIZE: 456 amino acids; 51802 Da
SUBUNIT: Binds UBQLN1 (By similarity). Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.
SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
DISEASE: SwissProt: P14867 # Defects in GABRA1 are a cause of juvenile myoclonic epilepsy (JME) [MIM:606904]. JME is a common epileptic syndrome characterized by afebrile seizures, onset in adolescence (rather than in childhood) and myoclonic jerks. & Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. ECA4 is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Absence seizures may either remit or persist into adulthood.
SIMILARITY: SwissProt: P14867 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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