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Milli-Mark™ Anti-mouse CD3-FITC clone 145-2C11

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货号：FCMAB227F

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M FC Ht FITC Monoclonal Antibody
Description
Catalogue Number FCMAB227F
Trade Name
  • Milli-Mark
Description Milli-Mark™ Anti-mouse CD3-FITC Antibody, clone 145-2C11
Alternate Names
  • T-cell surface glycoprotein CD3 epsilon chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • CD3e
Background Information Human TCR/CD3 is a complex structure on the lymphocyte surface. It consists of the TCR alpha beta or TCR gamma delta heterodimer and the associated CD3 complex. The CD3 complex is composed of six polypeptides with usually four different transmembrane CD3 chains, gamma, delta, epsilon. and zeta. Three different dimers, gamma epsilon, delta epsilon, and zeta zeta, constitute the CD3 complex. The MW of CD3epsilon is 20 000 (Campana, 1987). CD3 is first detectable in early thymocytes and its appearance probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, during early stages of maturation, the CD3 antigen is predominantly present in the cell cytoplasm. In medullary thymocyles, the CD3 antigen is predominantly detected on the cell surface (Swerdlow, 1988; Beverley, 1981). The majority of T cell neoplasms also expresses CD3, but it is absent from non-T-cell lymphoid malignancies (McMichael, 1987). Consistent with the pattern of synthesis of the antigen in normal thymocytes, the earliest site detectable within neoplastic cells is the cell cytoplasm (Swerdlow, 1988).
References
Product Information
Format FITC
Control
  • Mouse splenocytes
Presentation Purified hamster monoclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Applications
Application This Milli-Mark Anti-mouse CD3-FITC Antibody, clone 145-2C11 is validated for use in FC for the detection of mouse CD3.
Key Applications
  • Flow Cytometry
Biological Information
Immunogen Animals were immunized with the BM10-37 mouse cytotoxic T lymphocyte (CTL) cell clone (anti H-2 Kb). Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Clone 145-2C11
Host Hamster
Specificity Antibody recognizes CD3 epsilon chain.
Isotype IgG
Species Reactivity
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Cd3e
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: The CD3 complex mediates signal transduction.

SUBUNIT STRUCTURE: The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

SEQUENCE SIMILARITIES: Contains 1 Ig-like (immunoglobulin-like) domain. Contains 1 ITAM domain.
Molecular Weight 21 kDa Calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by flow cytometry using mouse splenocytes
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
Packaging Information
Material Size 100 tests
Transport Information
Supplemental Information
Specifications

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