RB X ANGPTL4 (MID)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

Purified

Polyclonal Antibody

Description
Catalogue Number	AB10605
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-ANGPTL4 (MID) Antibody
Background Information	ANGPTL4 (angiopoietin-like protein 4, hepatic fibrinogen/angiopoietin-related protein (HFARP), fasting-induced adipose factor (FIAF), PPARγ angiopoietin-related protein (PGAR)) is a secreted protein selectively expressed in adipose tissue, liver, and placent, that plays a variety of roles in vivo, ranging from adipogenesis to angiogenesis to carcinogenesis. Several transcription factors exert influence on ANGPTL4 transcription, including PPARα, PPARγ, and HIF 1α. PPARα and HIF 1α synergistically cause the activation of ANGPTL4 in cardiomyocytes; induction of ANGPTL4 in the heart inhibits lipoprotein-derived fatty acid delivery. As a transcriptional target of PPARγ, ANGPTL4 has been hypothesized to play a role in adipogenesis, insulin sensitivity, and energy metabolism. The expression of ANGPTL4 is also under nutritional and hormonal control. During fasting conditions, transcription of ANGPTL4 in both liver and adipose tissue is induced independently of PPARα1 While circulating levels of ANGPTL4 are increased in genetically obese mice, rodents fed a highfat diet demonstrate reduced circulating ANGPTL4 levels, suggesting that ANGPTL4 may be involved in response to the availability of nutrients. In endothelial cells, ANGPTL4 mRNA and protein levels increase in response to hypoxia. ANGPTL4 has been observed to induce a strong pro-angiogenic response independent of vascular endothelial growth factor (VEGF), and its expression has been described in hypoxic human tissues as well as a variety of cancers, including liposarcoma, hepatocellular carcinoma, and conventional renal cell carcinoma. Taken together, these findings suggest that ANGPTL4 may be involved in the mechanisms that compensate for ischemia by angiogenesis.

Description

Catalogue Number

AB10605

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-ANGPTL4 (MID) Antibody

Background Information

ANGPTL4 (angiopoietin-like protein 4, hepatic fibrinogen/angiopoietin-related protein (HFARP), fasting-induced adipose factor (FIAF), PPARγ angiopoietin-related protein (PGAR)) is a secreted protein selectively expressed in adipose tissue, liver, and placent, that plays a variety of roles in vivo, ranging from adipogenesis to angiogenesis to carcinogenesis. Several transcription factors exert influence on ANGPTL4 transcription, including PPARα, PPARγ, and HIF 1α. PPARα and HIF 1α synergistically cause the activation of ANGPTL4 in cardiomyocytes; induction of ANGPTL4 in the heart inhibits lipoprotein-derived fatty acid delivery. As a transcriptional target of PPARγ, ANGPTL4 has been hypothesized to play a role in adipogenesis, insulin sensitivity, and energy metabolism. The expression of ANGPTL4 is also under nutritional and hormonal control. During fasting conditions, transcription of ANGPTL4 in both liver and adipose tissue is induced independently of PPARα1 While circulating levels of ANGPTL4 are increased in genetically obese mice, rodents fed a highfat diet demonstrate reduced circulating ANGPTL4 levels, suggesting that ANGPTL4 may be involved in response to the availability of nutrients. In endothelial cells, ANGPTL4 mRNA and protein levels increase in response to hypoxia. ANGPTL4 has been observed to induce a strong pro-angiogenic response independent of vascular endothelial growth factor (VEGF), and its expression has been described in hypoxic human tissues as well as a variety of cancers, including liposarcoma, hepatocellular carcinoma, and conventional renal cell carcinoma. Taken together, these findings suggest that ANGPTL4 may be involved in the mechanisms that compensate for ischemia by angiogenesis.

Product Information
Format	Purified
Presentation	400 μL aliquot at a concentration of 0.25 mg/mL in phosphate buffered saline (pH 7.4) containing 0.1% sodium azide.

Product Information

Format

Purified

Presentation

400 μL aliquot at a concentration of 0.25 mg/mL in phosphate buffered saline (pH 7.4) containing 0.1% sodium azide.

Applications
Application	Anti-ANGPTL4 (MID) Antibody is an antibody against ANGPTL4 (MID) for use in WB.
Key Applications	Western Blotting
Application Notes	Western Blotting: 1-3 μg/mL

Applications

Application

Anti-ANGPTL4 (MID) Antibody is an antibody against ANGPTL4 (MID) for use in WB.

Key Applications

Western Blotting

Application Notes

Western Blotting: 1-3 μg/mL

Biological Information
Immunogen	Synthetic peptide derived from an internal region of the human ANGTPL4 protein, which differs from rat and mouse by one and two conservative amino acid replacements, respectively.
Clone	ZMD.521
Host	Rabbit
Specificity	Recognizes ANGPTL4 (MID).
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Human, Mouse, Rat (positive controls: HepG2 and NGP96 cell lysates, mouse placenta and rat liver homogenates, serum-stimulated mouse 3T3-L1 embryo fibroblast lysates, and 2 week-old mouse brain homogenates).
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_001039667.1 NM_139314.1
Entrez Gene Summary	This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. The encoded protein is a serum hormone directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity and also acts as an apoptosis survival factor for vascular endothelial cells. The encoded protein may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4.
Gene Symbol	ANGPTL4 UNQ171/PRO197 HFARP ANGPTL2 PSEC0166 PGAR NL2 pp1158 ARP4 FIAF
Purification Method	ImmunoAffinity Purified
UniProt Number	Q9BY76
UniProt Summary	FUNCTION: SwissProt: Q9BY76 # Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation (By similarity). SIZE: 406 amino acids; 45214 Da SUBUNIT: Homooligomer. The homooligomer undergoes proteolytic processing to release its carboxyl fibrinogen-like domain, which circulates as a monomer. The homooligomer unprocessed form is able to interact with the extracellular matrix (By similarity). SUBCELLULAR LOCATION: Secreted. Secreted, extracellular space, extracellular matrix (By similarity). Note=The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4 (By similarity).TISSUE SPECIFICITY: Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. PTM: N-glycosylated. DISEASE: SwissProt: Q9BY76 # Found to be highly expressed in the early stages of collagen-induced arthritis (CIA). & Serum levels of ANGPTL4 are significantly lower in patients with diabetes type 2 than those in healthy subjects, suggesting that decreased ANGPTL4 could be a causative factor of this disease. & Produced in ischemic tissues in conditions such as critical leg ischemia. In tumors, ANGPTL4 could be produced in the hypoxic areas surrounding necrotic regions. High levels could be produced in tumor cells of conventional renal cell carcinoma. This molecule therefore seems to be a marker of conventional renal cell carcinoma. SIMILARITY: SwissProt: Q9BY76 ## Contains 1 fibrinogen C-terminal domain.

Biological Information

Immunogen

Synthetic peptide derived from an internal region of the human ANGTPL4 protein, which differs from rat and mouse by one and two conservative amino acid replacements, respectively.

Clone

ZMD.521

Host

Rabbit

Specificity

Recognizes ANGPTL4 (MID).

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Human, Mouse, Rat (positive controls: HepG2 and NGP96 cell lysates, mouse placenta and rat liver homogenates, serum-stimulated mouse 3T3-L1 embryo fibroblast lysates, and 2 week-old mouse brain homogenates).

Antibody Type

Polyclonal Antibody

Entrez Gene Number

Entrez Gene Summary

This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. The encoded protein is a serum hormone directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity and also acts as an apoptosis survival factor for vascular endothelial cells. The encoded protein may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4.

Gene Symbol

ANGPTL4
UNQ171/PRO197
HFARP
ANGPTL2
PSEC0166
PGAR
NL2
pp1158
ARP4
FIAF

Purification Method

ImmunoAffinity Purified

UniProt Number

Q9BY76

UniProt Summary

FUNCTION: SwissProt: Q9BY76 # Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation (By similarity).
SIZE: 406 amino acids; 45214 Da
SUBUNIT: Homooligomer. The homooligomer undergoes proteolytic processing to release its carboxyl fibrinogen-like domain, which circulates as a monomer. The homooligomer unprocessed form is able to interact with the extracellular matrix (By similarity).
SUBCELLULAR LOCATION: Secreted. Secreted, extracellular space, extracellular matrix (By similarity). Note=The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4 (By similarity).TISSUE SPECIFICITY: Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney.
PTM: N-glycosylated.
DISEASE: SwissProt: Q9BY76 # Found to be highly expressed in the early stages of collagen-induced arthritis (CIA). & Serum levels of ANGPTL4 are significantly lower in patients with diabetes type 2 than those in healthy subjects, suggesting that decreased ANGPTL4 could be a causative factor of this disease. & Produced in ischemic tissues in conditions such as critical leg ischemia. In tumors, ANGPTL4 could be produced in the hypoxic areas surrounding necrotic regions. High levels could be produced in tumor cells of conventional renal cell carcinoma. This molecule therefore seems to be a marker of conventional renal cell carcinoma.
SIMILARITY: SwissProt: Q9BY76 ## Contains 1 fibrinogen C-terminal domain.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

浏览了该商品的用户最终购买

浏览了该商品的用户还浏览了

特卖

重要规格表

我要咨询