Ms X NG2

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	FC, WB	M	Ascites	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

FC, WB

Ascites

Monoclonal Antibody

Description
Catalogue Number	MAB5520
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1
Alternate Names	NG2

Description

Catalogue Number

MAB5520

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1

Alternate Names

Product Information
Format	Ascites
Presentation	Ascites fluid. Liquid. Contains no preservative.

Product Information

Format

Ascites

Presentation

Ascites fluid. Liquid. Contains no preservative.

Applications
Application	Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1 is an antibody against NG2 Chondroitin Sulfate Proteoglycan for use in FC & WB.
Key Applications	Flow Cytometry Western Blotting
Application Notes	Western blot Flow cytometry Optimal working dilutions must be determined by end user.

Applications

Application

Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1 is an antibody against NG2 Chondroitin Sulfate Proteoglycan for use in FC & WB.

Key Applications

Flow Cytometry
Western Blotting

Application Notes

Western blot

Flow cytometry

Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Human bone marrow stromal cell line, SV40 transformed.
Clone	7.1
Host	Mouse
Specificity	NG2 (chondroitin sulfate proteoglycan), human which is the homologue of the rat NG2 molecule. The antibody reacts with a 220-240 kD surface protein whose N-terminal sequence is identical to the rat NG2 chondrotin sulfate proteoglycan molecule. MAB5520 reacts with only neoplastic leukocytes with specific association with chromosome 11q23 translocations involving the MLL gene. It has a very high specificity for t(4;11) or t(11;19) translocations. The antibody shows no reactivity with normal marrow hematopoietic cells, peripheral blld leukocytes, red blood cells or platelets. Leukemic cells lines KG1, KG1a, HRL, Jurkat, K562, Raji, HL-60, U937, Daudi, Nalm6 and CEM are negative. However MAB5520 does react with a variety of cell lines of nonhematopoietic origin, including HeLa cells.
Isotype	IgG1
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001897.4
Entrez Gene Summary	A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Gene Symbol	CSPG4 MSK16 MCSP NG2 MCSPG MEL-CSPG HMW-MAA
UniProt Number	Q6UVK1
UniProt Summary	FUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. SIZE: 2322 amino acids; 250497 Da SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16. SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium (By similarity). Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity). TISSUE SPECIFICITY: Detected only in malignant melanoma cells. PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4. & Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity). SIMILARITY: SwissProt: Q6UVK1 ## Contains 15 CSPG (NG2) repeats. & Contains 2 laminin G-like domains. MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells.

Biological Information

Immunogen

Human bone marrow stromal cell line, SV40 transformed.

Clone

7.1

Host

Mouse

Specificity

NG2 (chondroitin sulfate proteoglycan), human which is the homologue of the rat NG2 molecule. The antibody reacts with a 220-240 kD surface protein whose N-terminal sequence is identical to the rat NG2 chondrotin sulfate proteoglycan molecule.

MAB5520 reacts with only neoplastic leukocytes with specific association with chromosome 11q23 translocations involving the MLL gene. It has a very high specificity for t(4;11) or t(11;19) translocations. The antibody shows no reactivity with normal marrow hematopoietic cells, peripheral blld leukocytes, red blood cells or platelets. Leukemic cells lines KG1, KG1a, HRL, Jurkat, K562, Raji, HL-60, U937, Daudi, Nalm6 and CEM are negative. However MAB5520 does react with a variety of cell lines of nonhematopoietic origin, including HeLa cells.

Isotype

IgG1

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_001897.4

Entrez Gene Summary

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

Gene Symbol

CSPG4
MSK16
MCSP
NG2
MCSPG
MEL-CSPG
HMW-MAA

UniProt Number

Q6UVK1

UniProt Summary

FUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.

SIZE: 2322 amino acids; 250497 Da

SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.

SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium (By similarity). Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity).

TISSUE SPECIFICITY: Detected only in malignant melanoma cells.

PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4. & Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity).

SIMILARITY: SwissProt: Q6UVK1 ## Contains 15 CSPG (NG2) repeats. & Contains 2 laminin G-like domains.

MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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