Ms X Integrin Beta 3

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	FC, FUNC, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

FC, FUNC, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	MAB2023Z
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Integrin β3 Antibody, clone B3A, azide free
Alternate Names	CD61 MAB2023

Description

Catalogue Number

MAB2023Z

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Integrin β3 Antibody, clone B3A, azide free

Alternate Names

CD61
MAB2023

Product Information
Format	Purified
Control	Positive Control: U251 or D54 human cell lines. Membrane preps are recommended for western blots. Human endothelium cells (expression of integrin alpha V beta 3 may need to be upregulated by phorbol esters stimulation). Skin (Basement Membrane)
Presentation	Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.

Product Information

Format

Purified

Control

Positive Control: U251 or D54 human cell lines. Membrane preps are recommended for western blots.
Human endothelium cells (expression of integrin alpha V beta 3 may need to be upregulated by phorbol esters stimulation). Skin (Basement Membrane)

Presentation

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.

Applications
Application	Anti-Integrin β3 Antibody, clone B3A, azide free detects level of Integrin β3 & has been published & validated for use in FC, FUNC & WB.
Key Applications	Flow Cytometry Affects Function Western Blotting
Application Notes	Flow cytometry Function-blocking of integrin beta3-mediated adhesion to ECM proteins. Western blot (reducing conditions) Optimal working dilutions must be determined by end user.

Applications

Application

Anti-Integrin β3 Antibody, clone B3A, azide free detects level of Integrin β3 & has been published & validated for use in FC, FUNC & WB.

Key Applications

Flow Cytometry
Affects Function
Western Blotting

Application Notes

Flow cytometry

Function-blocking of integrin beta3-mediated adhesion to ECM proteins.

Western blot (reducing conditions) Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Human integrin alphaVbeta3 heterodimer isolated from placenta.
Clone	B3A
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Specific for human integrin beta 3 (other species not tested).
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_000212.2
Entrez Gene Summary	The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
Gene Symbol	ITGB3 GP3A CD61 GPIIIa
Purification Method	Protein A Purfied
UniProt Number	P05106
UniProt Summary	FUNCTION: SwissProt: P05106 # Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. SIZE: 788 amino acids; 87058 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIb or alpha-V. Isoform Beta-3C interacts with FLNB. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis. PTM: Phosphorylated on tyrosine residues in response to thrombin- induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. SHC binding also occurs when Tyr-785 alone is phosphorylated. DISEASE: SwissProt: P05106 # Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. Its inheritance is autosomal recessive. It is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb-IIIa complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the GPIIb-IIIa complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT variants have normal or near normal (60-100%) expression of dysfunctional receptors. SIMILARITY: SwissProt: P05106 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
Molecular Weight	110 kDa

Biological Information

Immunogen

Human integrin alphaVbeta3 heterodimer isolated from placenta.

Clone

B3A

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Specific for human integrin beta 3 (other species not tested).

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_000212.2

Entrez Gene Summary

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

Gene Symbol

ITGB3
GP3A
CD61
GPIIIa

Purification Method

Protein A Purfied

UniProt Number

P05106

UniProt Summary

FUNCTION: SwissProt: P05106 # Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 788 amino acids; 87058 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIb or alpha-V. Isoform Beta-3C interacts with FLNB. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
PTM: Phosphorylated on tyrosine residues in response to thrombin- induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. SHC binding also occurs when Tyr-785 alone is phosphorylated.
DISEASE: SwissProt: P05106 # Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. Its inheritance is autosomal recessive. It is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb-IIIa complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the GPIIb-IIIa complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT variants have normal or near normal (60-100%) expression of dysfunctional receptors.
SIMILARITY: SwissProt: P05106 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.

Molecular Weight

110 kDa

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Storage and Shipping Information

Storage Conditions

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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