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Anti-IDO, clone 1F8.2 (mouse monoclonal)

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货号：MAB10009

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IHC M Purified Monoclonal Antibody
Description
Catalogue Number MAB10009
Description Anti-IDO Antibody, clone 1F8.2
Alternate Names
  • Indoleamine-pyrrole 2,3-dioxygenase
  • indole 2,3-dioxygenase
  • indoleamine 2,3-dioxygenase 1
  • indoleamine-pyrrole 2,3 dioxygenase
  • IDO1
Background Information Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the
suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.
References
Product Information
Format Purified
Control
  • HeLa cell lysate treated with interferon gamma.
Presentation Mouse monoclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.
Applications
Application Anti-IDO Antibody, clone 1F8.2 is an antibody against IDO for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Biological Information
Immunogen GST-tagged recombinant protein of full-length human IDO.
Clone 1F8.2
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity This antibody recognizes IDO.
Isotype IgG1κ
Species Reactivity
  • Human
Species Reactivity Note Proven to react with human.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD107B
  • EC 1.13.11.52
  • IDO
  • INDO
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
CATALYTIC ACTIVITY: D-tryptophan + O2 = N-formyl-D-kynurenine.
L-tryptophan + O2 = N-formyl-L-kynurenine.
COFACTOR: Binds 1 heme group per subunit.
INDUCTION: By interferon gamma.
SEQUENCE SIMILARITIES: Belongs to the indoleamine 2,3-dioxygenase family.
Molecular Weight Approx. 45 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL dilution of this antibody detected IDO on 15 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2-8°C for 1 year from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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