Anti-Emerin, clone 7D9 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, ICC

Purified

Monoclonal Antibody

Description
Catalogue Number	MABE69
Description	Anti-Emerin Antibody, clone 7D9
Alternate Names	Emery-Dreifuss muscular dystrophy LEM domain containing 5
Background Information	Emerin is a LEM domain-containing protein found in the nuclear membrane in vertebrates. The LEM domain, a conserved globular domain of approximately 40 amino acids, is present in many nuclear membrane associated proteins. Emerin is known to interact with nuclear lamins, barrier-to-autointegration factor (BAF), nesprin-1 alpha, and a transcription repressor. Functionally, this protein mediates membrane anchorage to the cytoskeleton, stabilizes and promotes formation of the nuclear actin cortical network, and inhibits beta-catenin activity by preventing nuclear accumulation. Mutations to emerin result in Dreifuss-Emery muscular dystrophy, an X-linked inherited degenerative myopathy. This protein appears to play a role in response to mechanical strain and the control of apoptosis due to strain. Emerin may also play a part in chromosome location and anchoring.

Description

Catalogue Number

MABE69

Description

Anti-Emerin Antibody, clone 7D9

Alternate Names

Emery-Dreifuss muscular dystrophy
LEM domain containing 5

Background Information

Emerin is a LEM domain-containing protein found in the nuclear membrane in vertebrates. The LEM domain, a conserved globular domain of approximately 40 amino acids, is present in many nuclear membrane associated proteins. Emerin is known to interact with nuclear lamins, barrier-to-autointegration factor (BAF), nesprin-1 alpha, and a transcription repressor. Functionally, this protein mediates membrane anchorage to the cytoskeleton, stabilizes and promotes formation of the nuclear actin cortical network, and inhibits beta-catenin activity by preventing nuclear accumulation. Mutations to emerin result in Dreifuss-Emery muscular dystrophy, an X-linked inherited degenerative myopathy. This protein appears to play a role in response to mechanical strain and the control of apoptosis due to strain. Emerin may also play a part in chromosome location and anchoring.

Product Information
Format	Purified
Control	HeLa cell lysate
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

HeLa cell lysate

Presentation

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Use Anti-Emerin Antibody, clone 7D9 (Mouse Monoclonal Antibody) validated in WB, ICC to detect Emerin also known as Emery-Dreifuss muscular dystrophy, LEM domain containing 5.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunocytochemistry Analysis: 1:50 dilution from a representative lot detected Emerin in normal human muscle cells. Courtesy of Dr. Ian Holt, Wolfson Centre for Inherited Neuromuscular Disease.

Applications

Application

Use Anti-Emerin Antibody, clone 7D9 (Mouse Monoclonal Antibody) validated in WB, ICC to detect Emerin also known as Emery-Dreifuss muscular dystrophy, LEM domain containing 5.

Key Applications

Western Blotting
Immunocytochemistry

Application Notes

Immunocytochemistry Analysis: 1:50 dilution from a representative lot detected Emerin in normal human muscle cells. Courtesy of Dr. Ian Holt, Wolfson Centre for Inherited Neuromuscular Disease.

Biological Information
Immunogen	Full length recombinant protein corresponding to human Emerin.
Clone	7D9
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000108
Entrez Gene Summary	Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq].
Gene Symbol	EDMD LEMD5 STA emerin
Purification Method	Protein G
UniProt Number	P50402
UniProt Summary	FUNCTION: Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C. SUBUNIT STRUCTURE: Interacts with lamins A and C, BANF1, GMCL, BCLAF1 and YTHDC1/YT521. Interacts with TMEM43; the interaction retains emerin in the nuclear inner membrane. Interacts with SUN1 and SUN2 (By similarity). Interacts with ACTB, SPTAN1, F-actin, CTNNB1 and beta-tubulin. SUBCELLULAR LOCATION: Nucleus inner membrane; Single-pass membrane protein; Nucleoplasmic side. Nucleus outer membrane. Note: Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus. TISSUE SPECIFICTY: Skeletal muscle, heart, colon, testis, ovary and pancreas. PTM: Found in four different phosphorylated forms, three of which appear to be associated with the cell cycle. INVOLVEMENT IN DISEASE: Defects in EMD are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1) [MIM:310300]. A degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. Sequence similarities Contains 1 LEM domain.
Molecular Weight	~29 kDa observed

Biological Information

Immunogen

Full length recombinant protein corresponding to human Emerin.

Clone

7D9

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Isotype

IgG1κ

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_000108

Entrez Gene Summary

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq].

Gene Symbol

EDMD
LEMD5
STA
emerin

Purification Method

Protein G

UniProt Number

P50402

UniProt Summary

FUNCTION: Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.

SUBUNIT STRUCTURE: Interacts with lamins A and C, BANF1, GMCL, BCLAF1 and YTHDC1/YT521. Interacts with TMEM43; the interaction retains emerin in the nuclear inner membrane. Interacts with SUN1 and SUN2 (By similarity). Interacts with ACTB, SPTAN1, F-actin, CTNNB1 and beta-tubulin.

SUBCELLULAR LOCATION: Nucleus inner membrane; Single-pass membrane protein; Nucleoplasmic side. Nucleus outer membrane. Note: Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus.

TISSUE SPECIFICTY: Skeletal muscle, heart, colon, testis, ovary and pancreas.

PTM: Found in four different phosphorylated forms, three of which appear to be associated with the cell cycle.

INVOLVEMENT IN DISEASE: Defects in EMD are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1) [MIM:310300]. A degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects.

Sequence similarities Contains 1 LEM domain.

Molecular Weight

~29 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Emerin on 10 µg of HeLa cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Emerin on 10 µg of HeLa cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Emerin, clone 7D9 (mouse monoclonal)

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