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Anti-p73 (rabbit polyclonal)

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货号：AB7824

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H IP, WB, IH(P) Rb Purified Polyclonal Antibody
Description
Catalogue Number AB7824
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-p73 Antibody, α and β
Alternate Names
  • p53-like Transcription Factor
References
Product Information
Format Purified
Presentation Purified immunoglobulin. 0.22 micron filtered. Provided at 1 mg/mL in PBS, pH 7.4, containing 0.05% sodium azide.
Applications
Application Anti-p73 Antibody, α & β is a Rabbit Polyclonal Antibody for detection of p73 also known as p53-like Transcription Factor & has been validated in IP, WB, IHC(P).
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application Notes Western blot: 1 μg/mL of the antibody will detect p73 in 30μg of total cell lysate from overexpressing cell lines. Higher levels of the antibody may be necessary when using native tissue sources.

Immunoprecipitation: Add 1μg of antibody to a sample that has been precleared with Protein A Sepharose™ in 500 μl lysis buffer. Incubate antibody with sample at 4°C for 2 hours with agitation. Follow by incubation with 50 μL Protein A Sepharose™ for 2 hours at 4°C with agitation. Collect the Protein A complex by centrifugation, washing pellet 3 times in lysis buffer. Resuspend final sample in electrophesis buffer, denature by heating at 95°C for 5 minutes, and analyze by SDS-PAGE

Immunohistochemistry of paraffin sections: Signal amplification may be necessary, in conjunction with standard IHC procedures. Dewax and rehydrate sections through descending graded alcohols to water using standard dewaxing protocols. Subject section to heat antigen retrieval. Wash sections 3 times with TBS, pH 7.4. Block endogenous peroxidase activity by incubating the section in 1% H2O2 in methanol for 30 minutes. Wash with TBS and detect according to preferred detection methods. With high sensitivity detection systems, typical working dilutions for the p73 antibody are 1:5,000 - 1;10,000.

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen GST fusion protein containing aa450-636 of p73 protein.
Epitope alpha and beta
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Antibody AB7824 recognizes amino acids 450-636 of p73 protein. This region is common to both p73alpha and p73beta isoforms, hence both isoforms of the p73 protein will be detected.
Species Reactivity
  • Human
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TP73
  • p73
  • P73
UniProt Number
UniProt Summary FUNCTION: SwissProt: O15350 # Participates in the apoptotic response to DNA damage. When overproduced, activates transcription from p53-responsive promoters and induces apoptosis. May be a tumor suppressor protein.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
SIZE: 636 amino acids; 69623 Da
SUBUNIT: The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Isoform Beta interacts homotypically and with p53/TP53, whereas isoform Alpha does not. Isoform Gamma interacts homotypically and with all p73 isoforms. Isoform Delta interacts with isoform Gamma, isoform Alpha, and homotypically. Isoforms Alpha and Beta interact with HIPK2. Isoform Alpha interacts with RANBP9. Found in a complex with p53/TP53 and CABLES1. Isoform Beta interacts with WWOX. Interacts with HECW2.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas.
DOMAIN: SwissProt: O15350 Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain. & The WW-binding motif mediates interaction with WWOX.
PTM: Isoform alpha (but not isoform beta) is sumoylated on Lys- 627, which potentiates proteasomal degradation but does not affect transcriptional activity.DISEASE:SwissProt: O15350 # Maps to a chromosome region frequently mutated in diverse cell lines of human cancer. Appears not to be frequently mutated in human cancers, in contrast to p53. Hemizygosity is observed in neuroblastoma and oligodendroglioma.
SIMILARITY: Belongs to the p53 family.
MISCELLANEOUS: Activated and stabilized by interaction with RANBP9.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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