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Anti-Syntaxin (mouse monoclonal)

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货号：MAB325

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, Ht, Po ELISA, WB, IHC M Ascites Monoclonal Antibody
Description
Catalogue Number MAB325
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Syntaxin Antibody, clone SP7
Alternate Names
  • Syntaxin 1A
  • Neuron-specific Antigen HPC-1
  • STX1A
References
Product Information
Format Ascites
Presentation Liquid
Applications
Application Anti-Syntaxin Antibody, clone SP7 is an antibody against Syntaxin for use in ELISA, WB, IH.
Key Applications
  • ELISA
  • Western Blotting
  • Immunohistochemistry
Application Notes Immunohistochemistry:1:10

Formalin fixed (fixed <4 weeks)

Immunoblotting: 1:10

ELISA: 1:10

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Crude synaptic immunoprecipitate (human).
Clone SP7
Host Mouse
Specificity Syntaxin (34-36 kDa). Reacts with syntaxin expressed in bacterial systems. Useful to investigators who transplant human neurons or human neural derived cell lines into rodents since the antibody reacts with human but poorly with rat syntaxin. Also useful in double labeling studies due to the fact it is an IgG2a subclass monoclonal.
Isotype IgG2a
Species Reactivity
  • Human
  • Hamster
  • Pig
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Syntaxins function in this vesicle fusion process. Syntaxins also serve as a substrate for botulinum neurotoxin type C, a metalloprotease that blocks exocytosis and has high affinity for a molecular complex that includes the alpha-latrotoxin receptor (MIM 600565) which produces explosive exocytosis (Zhang et al., 1995 [PubMed 7622072]).[supplied by OMIM]
Gene Symbol
  • STX1A
  • HPC-1
  • STX1
  • Syntaxin-1A
  • p35-1
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q16623 # Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis.
SIZE: 288 amino acids; 33023 Da
SUBUNIT: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Binds SYTL4 and STXBP6. Found in a ternary complex with STX1A and SNAP25 (By similarity). Found in a complex with VAMP8 and SNAP23. Interacts with VAPA and SYBU.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type IV membrane protein. & Isoform 2: Secreted (Probable).
TISSUE SPECIFICITY: Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
DISEASE: SwissProt: Q16623 # Haploinsufficiency of STX1A may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.
SIMILARITY: SwissProt: Q16623 ## Belongs to the syntaxin family. & Contains 1 t-SNARE coiled-coil homology domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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