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Anti-ZO-1 (rabbit polyclonal)

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货号：AB2272

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, IH(P) Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB2272
Description Anti-ZO-1 Antibody
Alternate Names
  • tight junction protein 1
  • zonula occludens 1 protein
  • Zona occludens protein 1
  • Zonula occludens protein 1
  • zona occludens 1
Background Information Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane associated guanylate kinase (MAGUK) family and include occludin and zona occludin family members ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.
References
Product Information
Format Affinity Purified
Control
  • HepG2 cell lysate
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Detect ZO-1 using this Anti-ZO-1 Antibody validated for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application Notes Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected ZO-1 in normal human testis tissue.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to human ZO-1.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Species Reactivity
  • Human
  • Mouse
Species Reactivity Note Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
Other homologies: Rat ( 89% sequence homology).
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq].
Gene Symbol
  • ZO-1
  • ZO1
  • TJP1
Purification Method Affinity Purfied
UniProt Number
UniProt Summary FUNCTION: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.

SUBUNIT STRUCTURE: Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1 By similarity. Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with occludin, claudins, CGN/cingulin, CXADR, GJA12, GJD3 and UBN1.

SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction › tight junction. Note: Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.

TISSUE SPECIFICTY: The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

DOMAIN: The second PDZ domain mediates interaction with GJA12 (By similarity).

SEQUENCE SIMILARITIES: Belongs to the MAGUK family.

Contains 1 guanylate kinase-like domain.

Contains 3 PDZ (DHR) domains.

Contains 1 SH3 domain.

Contains 1 ZU5 domain.

SEQUENCE CAUTION: The sequence AAA02891.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Molecular Weight ~260 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected ZO-1 on 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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