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Rb X APG10

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货号：AB15408

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, ICC, IHC Rb Serum Polyclonal Antibody
Description
Catalogue Number AB15408
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-APG10 Antibody
Alternate Names
  • Autophagy-related protein 10
  • APG10-like
  • ATG10
Background Information Autophagy is the process by which cells recycle cytoplasm and dispose of excess or defective organelles. This process is suggested to be involved development, differentiation, growth regulation and tissue remodeling in multicellular organisms. The APG10 protein plays a role in the conjugation of APG5 to APG12 and this conjugate is required for elongation of the isolation membrane to form a complete spherical autophagosome. The APG10 is able to interact with MAP1LC3A and with APG5 and APG7.
References
Product Information
Format Serum
Presentation Lyophilized. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any insoluble material. Contains no preservative.
Applications
Application Anti-APG10 Antibody detects level of APG10 & has been published & validated for use in WB, IC, IH.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application Notes Western blot: 1:200-1:1,000
Immunocytochemistry: 1:200-1:1,000
Immunohistochemistry: 1:200-1:1,000
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Synthetic peptide corresponding to a region within the C-terminus of human APG10 protein.
Host Rabbit
Specificity APG10 [Autophagy-related protein 10, APG10-like, ATG10].
Species Reactivity
  • Human
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM]
Gene Symbol
  • ATG10
  • APG10
  • DKFZP586I0418
  • APG10L
  • APG10-like
  • ORFNames=PP12616
  • FLJ13954
  • pp12616
Purification Method Serum
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q9H0Y0 # Plays a role in autophagy (By similarity). ATG12- conjugating enzyme (E2-like enzyme), likely serves as an ATG5- recognition molecule.
SIZE: 220 amino acids; 25279 Da
SUBUNIT: Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: SwissProt: Q9H0Y0 ## Belongs to the ATG10 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material frozen at -20°C to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1, ACS or better grade) can be added for additional stability.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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