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Anti-SRp55 (rabbit polyclonal)

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货号：06-1366

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IP Rb Purified Polyclonal Antibody
Description
Catalogue Number 06-1366
Description Anti-SRp55 Antibody
Alternate Names
  • serine/arginine-rich splicing factor 6
  • splicing factor, arginine/serine-rich 6
  • arginine/serine-rich splicing factor 6
  • splicing factor, arginine/serine-rich, 55 kDa
  • Pre-mRNA-splicing factor SRP55
  • pre-mRNA splicing factor SRP55
  • SR splicing factor 6
Background Information Pre-mRNA-splicing factor SRp55, also known as Serine/arginine-rich splicing factor 6, is a member of the serine/arginine-rich (SR) protein family. This group of proteins is found mainly in the nucleus where they play a critical role in regulated and constitutive splicing of precursor mRNAs. SRp55 is involved in the constitutive process for mRNA splicing, and is able to mediate alternative splice site selection. Considered one of the more ubiquitous of the splice factors, SRp55 is upregulated in response to DNA damage when p53 is lacking. SRp55 is significantly phosphorylated on RS domain serine residues.
References
Product Information
Format Purified
Control
  • HeLa cell lysate
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-SRp55 Antibody is a Rabbit Polyclonal Antibody for detection of SRp55 also known as serine/arginine-rich splicing factor 6, Pre-mRNA-splicing factor SRP55 & has been validated in WB & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application Notes Immunoprecipitation Analysis:
A representative lot was used by an independent laboratory in IP. (Jiang, K., et al. (2009). Endocrinology. 150(5):2087-2097.)
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to human SRp55.
Host Rabbit
Species Reactivity
  • Human
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is involved in mRNA splicing and may play a role in the determination of alternative splicing. The encoded nuclear protein belongs to the splicing factor SR family and has been shown to bind with and modulate another member of the family, SFRS12. Alternative splicing results in multiple transcript variants. In addition, two pseudogenes, one on chromosome 17 and the other on the X chromosome, have been found for this gene.
Gene Symbol
  • SRP55
  • SFRS6
  • SRSF6
  • B52
Purification Method Protein A
UniProt Number
UniProt Summary FUNCTION: Plays a role in constitutive splicing and can modulate the selection of alternative splice sites.

SUBUNIT STRUCTURE: Binds SFRS12.

SUBCELLULAR LOCATION: Nucleus.

PTM: Extensively phosphorylated on serine residues in the RS domain (By similarity).

SEQUENCE SIMILARITIES: Belongs to the splicing factor SR family.

Contains 2 RRM (RNA recognition motif) domains.
Molecular Weight ~50 kDa observed. Mulitple uncharacterized bands have been observed in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected SRp55 on 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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