Anti-WNK4 (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	07-2263
Description	Anti-WNK4 Antibody
Alternate Names	WNK lysine deficient protein kinase 4 protein kinase, lysine deficient 4 Protein kinase with no lysine 4 serine/threonine-protein kinase WNK4 Protein kinase lysine-deficient 4
Background Information	WNK4 is a serine-threonine protein kinase that is commonly expressed in the distal nephron. Mutations in WNK1 and WNK4 cause pseudohypoaldosteronism type II, which causes hypertension, hyperkalemia, hyperchloremia and metabolic acidosis. WNK4 has been shown to decrease Na-Cl cotransporter activity and the potassium channel by removing it from the cell surface. It is thought that activating WNK4 inhibits the activity of WNK1. WNK4 has been shown to play a role in regulating blood pressure in addition to potassium homeostasis.

Description

Catalogue Number

07-2263

Description

Anti-WNK4 Antibody

Alternate Names

WNK lysine deficient protein kinase 4
protein kinase, lysine deficient 4
Protein kinase with no lysine 4
serine/threonine-protein kinase WNK4
Protein kinase lysine-deficient 4

Background Information

WNK4 is a serine-threonine protein kinase that is commonly expressed in the distal nephron. Mutations in WNK1 and WNK4 cause pseudohypoaldosteronism type II, which causes hypertension, hyperkalemia, hyperchloremia and metabolic acidosis. WNK4 has been shown to decrease Na-Cl cotransporter activity and the potassium channel by removing it from the cell surface. It is thought that activating WNK4 inhibits the activity of WNK1. WNK4 has been shown to play a role in regulating blood pressure in addition to potassium homeostasis.

Product Information
Format	Affinity Purified
Control	HEK293 cell lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

HEK293 cell lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	This WNK4 antibody is validated for use in WB for the detection of the WNK4 protein.
Key Applications	Western Blotting

Applications

Application

This WNK4 antibody is validated for use in WB for the detection of the WNK4 protein.

Key Applications

Western Blotting

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to the N-terminus of human WNK4.
Epitope	N-terminus
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes WNK4 at the N-terminus.
Species Reactivity	Human
Species Reactivity Note	Demonstrated to react with human. Other homologies: Rat (94% sequence homology).
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_115763
Entrez Gene Summary	This gene encodes a member of the WNK family of serine-threonine protein kinases. The kinase is part of the tight junction complex in kidney cells, and regulates the balance between NaCl reabsorption and K(+) secretion. The kinase regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. Mutations in this gene result in pseudohypoaldosteronism type IIB.
Gene Symbol	PHA2B WNK4 PRKWNK4
Purification Method	Affinity Purfied
UniProt Number	Q96J92
UniProt Summary	FUNCTION: Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K+ channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K+ secretion to maintain integrated homeostasis (By similarity). UniProtKB Q80UE6 CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Magnesium (By similarity). UniProtKB Q9H4A3 ENZYME REGULATION: Activation requires autophosphorylation of Ser-335. Phosphorylation of Ser-331 also promotes increased activity (By similarity). UniProtKB Q9JIH7 SUBUNIT STRUCTURE: Interacts with the C-terminal region of KCNJ1 By similarity. UniProtKB Q80UE6 SUBCELLULAR LOCATION: Cell junction › tight junction (By similarity). Note: Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct. WNK4 is part of the tight junction complex (By similarity). TISSUE SPECIFICTY: Expressed in kidney, colon and skin. Ref.4 INVOLVEMENT IN DISEASE: Defects in WNK4 are a cause of pseudohypoaldosteronism type II (PHAII) [MIM:145260]. PHAII is an autosomal dominant disease characterized by severe hypertension, hyperkalemia, and sensitivity to thiazide diuretics which may result from a chloride shunt in the renal distal nephron. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. Contains 1 protein kinase domain. CAUTION: Cys-203 is present instead of the conserved Lys which is expected to be an active site residue. Lys-186 appears to fulfill the required catalytic function. UniProtKB Q9JIH7 SEQUENCE CUTION: The sequence BAC04669.1 differs from that shown. Reason: Erroneous initiation. The sequence CAC48387.1 differs from that shown. Reason: Frameshift at position 4.
Molecular Weight	~150 kDa observed. .This protein has 3 isoforms produced by alternative splicing with molecular weights of 134, 73 & 126. Hengl, T., et al. (2010). PNAS. 107(13):6052–6057.

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminus of human WNK4.

Epitope

N-terminus

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes WNK4 at the N-terminus.

Species Reactivity

Human

Species Reactivity Note

Demonstrated to react with human. Other homologies: Rat (94% sequence homology).

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_115763

Entrez Gene Summary

This gene encodes a member of the WNK family of serine-threonine protein kinases. The kinase is part of the tight junction complex in kidney cells, and regulates the balance between NaCl reabsorption and K(+) secretion. The kinase regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. Mutations in this gene result in pseudohypoaldosteronism type IIB.

Gene Symbol

PHA2B
WNK4
PRKWNK4

Purification Method

Affinity Purfied

UniProt Number

Q96J92

UniProt Summary

FUNCTION: Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K+ channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K+ secretion to maintain integrated homeostasis (By similarity). UniProtKB Q80UE6

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

COFACTOR: Magnesium (By similarity). UniProtKB Q9H4A3

ENZYME REGULATION: Activation requires autophosphorylation of Ser-335. Phosphorylation of Ser-331 also promotes increased activity (By similarity). UniProtKB Q9JIH7

SUBUNIT STRUCTURE: Interacts with the C-terminal region of KCNJ1 By similarity. UniProtKB Q80UE6

SUBCELLULAR LOCATION: Cell junction › tight junction (By similarity). Note: Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct. WNK4 is part of the tight junction complex (By similarity).

TISSUE SPECIFICTY: Expressed in kidney, colon and skin. Ref.4

INVOLVEMENT IN DISEASE: Defects in WNK4 are a cause of pseudohypoaldosteronism type II (PHAII) [MIM:145260]. PHAII is an autosomal dominant disease characterized by severe hypertension, hyperkalemia, and sensitivity to thiazide diuretics which may result from a chloride shunt in the renal distal nephron.

SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.

Contains 1 protein kinase domain.

CAUTION: Cys-203 is present instead of the conserved Lys which is expected to be an active site residue. Lys-186 appears to fulfill the required catalytic function. UniProtKB Q9JIH7

SEQUENCE CUTION: The sequence BAC04669.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAC48387.1 differs from that shown. Reason: Frameshift at position 4.

Molecular Weight

~150 kDa observed. .This protein has 3 isoforms produced by alternative splicing with molecular weights of 134, 73 & 126. Hengl, T., et al. (2010). PNAS. 107(13):6052–6057.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HEK293 cell lysate. Western Blot Analysis: 1:500 dilution of this antibody detected WNK4 on 10 µg of HEK293 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected WNK4 on 10 µg of HEK293 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-WNK4 (rabbit polyclonal)

浏览了该商品的用户最终购买

浏览了该商品的用户还浏览了

特卖

重要规格表

我要咨询