Anti-End-binding protein 3 (EB3) (rabbit polyclonal)

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Anti-End-binding protein 3 (EB3) (rabbit polyclonal)

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货号：AB6033

品牌：Millipore 密理博

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Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Mk	WB, ICC, IP	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	AB6033
Description	Anti-End-binding protein 3 (EB3) Antibody
Alternate Names	microtubule-associated protein, RP/EB family, member 3 EB1 protein family member 3 microtubule-associated protein RP/EB family member 3 End-binding protein 3 APC binding protein
Background Information	EB (End-binding) proteins are evolutionarily conserved and are important for extending tips of microtubules as well as regulating microtubule dynamics and communication with other intracellular substances. End-binding protein 3 (also known as EB3 or Microtubule-associated protein RP/EB family member 3) is a 282 amino acid long protein that is 53% homologous to EB1, which is known to associate with adenomatous polyposis coli tumor suppressor. EB3 has been shown to prefer brain tissue. EB3 is highly expressed during myogenic differentiation and the loss of EB3 activity leads to the inability of myoblasts to elongate and combine into microtubules. EB3 may be involved in microtubule polymerization and cell migration. EB3 may be also involved in stabilizing microtubules and anchoring them to centrosomes during spindle formation.

References

Product Information
Format	Affinity Purified
Control	MCF-7 cell lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Detect End-binding protein 3 (EB3) using this Anti-End-binding protein 3 (EB3) Antibody validated for use in WB & IC.
Key Applications	Western Blotting Immunocytochemistry Immunoprecipitation
Application Notes	Immunocytochemistry Analysis: A previous lot was used by an independent laboratory to detect EB3 in MCF-7 cells (Dr. Anna Akhmanova, Erasmus Medical Center). Immunoprecipitation Analysis: A previous lot of this antibody immunoprecipitated EB3 from COS-7 cell lysates (Worth, D.C., et al. (2013) J. Cell Biol. Vol. 202 No. 5 793–806).

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to human EB3.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Detects EB3, but may show slight cross reactivity with EB1.
Species Reactivity	Human Monkey
Species Reactivity Note	Demonstrated to react with human. EB1 (67% sequence homology). Mouse EB3 (99% sequence homology). Rat EB3 (98% sequence homology).
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_036458
Entrez Gene Summary	The protein encoded by this gene is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene. [provided by RefSeq].
Gene Symbol	EB3 RP3 EBF3-S2 EBF3 MAPRE3
Purification Method	Affinity Purfied
UniProt Number	Q9UPY8
UniProt Summary	FUNCTION: May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity). SUBUNIT STRUCTURE: Interacts with APC2 (By similarity). Interacts with DCTN1 and SRCIN1. Binds to the C-terminal domain of APC. Binds monomeric and polymerized tubulin. SUBCELLULAR LOCATION: Cytoplasm. Note: Associated with the microtubule network. Ref.1 DOMAIN: Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1. SEQUENCE SIMILARITIES: Belongs to the MAPRE family. Contains 1 CH (calponin-homology) domain. Contains 1 EB1 C-terminal domain. SEQUENCE CAUTION: The sequence AAK07556.1 differs from that shown. Reason: Erroneous initiation. The sequence AAK07557.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA72060.1 differs from that shown. Reason: Frameshift at position 199.
Molecular Weight	~32 kDa observed

Physicochemical Information

Dimensions

Materials Information

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Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in MCF-7 cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected EB3 in 10 µg of MCF-7 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

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Anti-End-binding protein 3 (EB3) (rabbit polyclonal)

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