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Anti-EB1 (rabbit polyclonal)

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货号：AB6057

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ICC Rb Purified Polyclonal Antibody
Description
Catalogue Number AB6057
Description Anti-EB1 Antibody
Alternate Names
  • microtubule-associated protein, RP/EB family, member 1
  • adenomatous polyposis coli-binding protein EB1
  • microtubule-associated protein RP/EB family member 1
  • End-binding protein 1
  • APC-binding protein EB1
Background Information End binding protein 1 (EB1) is thought to be associated with adenomatous polyposis coli (APC) and may play a part in regulation of microtubule dynamics in the mammalian cells. EB1 could also play a role in the regulation of microtubule assembly and stability. EB1 is essential for proper cilia assembly in fibroblasts. EB1 has been shown to excite spontaneous nucleation and microtubule growth. APC gene mutation and the loss of negatively regulating WNT signaling is thought to play a role in the proliferation changes of cancer. The loss of association with EB1 could contribute to the development of certain cancers.
References
Product Information
Format Purified
Control
  • HeLa cell lysate
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-EB1 Antibody is an antibody against EB1 for use in WB & IC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application Notes Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected EB1 in NIH/3T3, A431, and HeLa cells.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to the C-terminus of human EB1.
Epitope C-terminus
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity This antibody recognizes EB1 at the C-terminus.
EB1 is 67% homologous to EB3.
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity Note Demonstrated to react with human.
Predicted to react with mouse and rat based on 100% sequence homology.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq].
Gene Symbol
  • EB1
  • MAPRE1
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.

SUBUNIT STRUCTURE: Homodimer. Interacts with DCTN1, DCTN2, DIAPH1, DIAPH2, TERF1 and dynein intermediate chain. Interacts with APC (via C-terminal domain), CLASP2, DST, KIF2C and STIM1; probably required for their targeting to the growing microtubule plus ends. Interacts with MTUS2; interaction is direct and probably targets MTUS2 to microtubules. Interacts with APC2. Interacts with CLASP1. Interacts with CDK5RAP2.

SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Cytoplasm › cytoskeleton › centrosome. Note: Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome.

TISSUE SPECIFICTY: Ubiquitously expressed.

DOMAIN: Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.

SEQUENCE SIMILARITIES: Belongs to the MAPRE family.

Contains 1 CH (calponin-homology) domain.

Contains 1 EB1 C-terminal domain.
Molecular Weight ~32 kDa observed.
An uncharacterized band appears at ~52 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected EB1 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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