Anti-a-Tubulin, clone DM1A, Alexa Fluor® 488 Conjugate

您好！欢迎来到炼石商城请登录注册兼职小伙伴

我的购物车

Qiagen 凯杰 Biosharp Omega

客服热线 0771-3293894

首页抗体Millipore 密理博>

图片仅供参考，请以实物为准

Anti-a-Tubulin, clone DM1A, Alexa Fluor® 488 Conjugate

市场价：: 0.00

促销价：: ¥0.00

货号：16-232

品牌：Millipore 密理博

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计3-5工作日到货)

亲，想了解价格请QQ咨询客服！

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

: 客服郭恒蔡玉坤曾宪飞技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次

3720.00

Plasmid Mini Kit I(200) 200...

576.00

10ul吸头，透明，袋装，可灭菌...

85.80

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (...

3000.00

QIAamp DNA Blood Midi Kit (2...

2680.00

QIAamp UCP Pathogen Mini Kit...

4112.00

商品介绍
商品咨询

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R, Gp, B, M, Po, Av	FC, ICC, IF, WB	M	AlexaFluor®488	Monoclonal Antibody

Description
Catalogue Number	16-232
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 488 conjugate
Alternate Names	Alpha-tubulin 3 Tubulin B-alpha-1 Tubulin alpha-3 chain tubulin, alpha 1a tubulin, alpha 3 tubulin, alpha, brain-specific
Background Information	Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.

References

Product Information
Format	AlexaFluor®488
Control	Included Negative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor® 488-conjugate.
Presentation	Alexa Fluor® 488 conjugated, purified mouse monoclonal IgG1 in buffer containing PBS containing 1% BSA, 0.05% Tween®-20, 0.05% sodium azide. Liquid at 2-8°C.

Applications
Application	Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate is an antibody against α-Tubulin for use in FC, IC, IF & WB.
Key Applications	Flow Cytometry Immunocytochemistry Immunofluorescence Western Blotting
Application Notes	Western Blot Analysis: 2 µg/mL of a previous lot detected α-Tubulin in RIPA lysates from A431 cells. Immunocytochemistry: 2 µg/mL of a previous lot showed positive immunostaining for α-Tubulin in HeLa cells.

Biological Information
Immunogen	Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).
Epitope	The epitopehas been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).
Clone	DM1A
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Recognizes α-Tubulin, clone DM1A, Mr 50-60 kDa.
Isotype	IgG1
Species Reactivity	Human Rat Guinea Pig Bovine Mouse Pig Avian
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_006000
Entrez Gene Summary	Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.
Gene Symbol	TUBA1A B-ALPHA-1 FLJ25113 hum-a-tub1 TUBA3 hum-a-tub2
Purification Method	Protein G Purified
UniProt Number	Q71U36
UniProt Summary	FUNCTION:SwissProt: Q71U36 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SIZE:451 amino acids; 50136 Da SUBUNIT: Dimer of alpha and beta chains. PTM:Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively (By similarity). SIMILARITY:SwissProt: Q71U36 ## Belongs to the tubulin family.
Molecular Weight	50-60 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Flow Cytometry. Flow Cytometry: 0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6, Jurkat, and A431 cells.
Usage Statement	Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Flow Cytometry.

Flow Cytometry:
0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6, Jurkat, and A431 cells.

Usage Statement

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt. Do Not Freeze. Do not store the material diluted. For maximum recovery of product, centrifuge original vial prior to removing cap.