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Anti-phospho-Acetyl CoA Carboxylase (Ser79), clone BK102

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货号：05-673

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M, R WB M Purified Monoclonal Antibody
Description
Catalogue Number 05-673
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody, clone BK102
References
Product Information
Format Purified
Control
  • Acid extracts of HeLa cells
Presentation Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl and 0.05% sodium azide, pH 7.4.
Applications
Application Detect phospho-Acetyl CoA Carboxylase (Ser79) using this Anti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody, clone BK102 validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen Synthetic peptide corresponding to rat Acetyl CoA Carboxylase
Clone BK102
Host Mouse
Specificity Recognizes phosphorylated Acetyl CoA Carboxylase (Ser79).
Isotype IgG1λ
Species Reactivity
  • Mouse
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
Gene Symbol
  • ACACA
  • ACC-alpha
  • ACCA
  • ACC
  • ACC1
  • ACAC
Modifications
  • Phosphorylation
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q13085 # Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
COFACTOR: Biotin. & Binds 2 manganese ions per subunit.
SIZE: 2346 amino acids; 265554 Da
SUBUNIT: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1.
SIMILARITY: SwissProt: Q13085 ## Contains 1 ATP-grasp domain. & Contains 1 biotin carboxylation domain. & Contains 1 biotinyl-binding domain. & Contains 1 carboxyltransferase domain.
Molecular Weight 257 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by immunoblot on HeLa acid extracts
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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