Anti-MAVS/Cardif, N-terminus

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IP	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, IP

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	06-1043
Description	Anti-MAVS/Cardif Antibody, NT
Alternate Names	mitochondrial antiviral signaling protein IFN-B promoter stimulator 1 CARD adaptor inducing IFN-beta virus-induced signaling adapter variant 1b Cardif Interferon beta promoter stimulator protein 1 CARD adapter inducing interferon-beta Putative NF-kappa-B-activating protein 031N interferon-beta promoter stimulator protein 1 Virus-induced-signaling adapter mitochondrial antiviral-signaling protein CARD adapter inducing interferon beta virus-induced signaling adaptor variant 1a CARDIF mitochondrial anti-viral signaling protein
Background Information	Mitochondrial antiviral-signaling protein (MAVS) is thought to activate NF-KappaB and IRF 3, which increase antiviral immunity. This result seems to point to mitochondrial involvement in innate immunity, specifically during viral infection. MAVS also seems to be involved in the activation of interferons, but only during viral contamination. It has been shown that MAVS works in conjunction with retinoic acid inducible gene I (RIG-I) to identify and remove replicating viral genomes. MAVS has been shown to be cleaved during apoptosis and viral infection, which could mean that this is an essential step of infection.

Description

Catalogue Number

06-1043

Description

Anti-MAVS/Cardif Antibody, NT

Alternate Names

mitochondrial antiviral signaling protein
IFN-B promoter stimulator 1
CARD adaptor inducing IFN-beta
virus-induced signaling adapter variant 1b
Cardif
Interferon beta promoter stimulator protein 1
CARD adapter inducing interferon-beta
Putative NF-kappa-B-activating protein 031N
interferon-beta promoter stimulator protein 1
Virus-induced-signaling adapter
mitochondrial antiviral-signaling protein
CARD adapter inducing interferon beta
virus-induced signaling adaptor variant 1a
CARDIF
mitochondrial anti-viral signaling protein

Background Information

Mitochondrial antiviral-signaling protein (MAVS) is thought to activate NF-KappaB and IRF 3, which increase antiviral immunity. This result seems to point to mitochondrial involvement in innate immunity, specifically during viral infection. MAVS also seems to be involved in the activation of interferons, but only during viral contamination. It has been shown that MAVS works in conjunction with retinoic acid inducible gene I (RIG-I) to identify and remove replicating viral genomes. MAVS has been shown to be cleaved during apoptosis and viral infection, which could mean that this is an essential step of infection.

Product Information
Format	Affinity Purified
Control	A549 cell lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

A549 cell lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-MAVS/Cardif Antibody, N-terminus is an antibody against MAVS/Cardif for use in WB & IP.
Key Applications	Western Blotting Immunoprecipitation
Application Notes	Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Lad, S., et al. (2008). Molecular Immunology. 45:2277–2287.)

Applications

Application

Anti-MAVS/Cardif Antibody, N-terminus is an antibody against MAVS/Cardif for use in WB & IP.

Key Applications

Western Blotting
Immunoprecipitation

Application Notes

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Lad, S., et al. (2008). Molecular Immunology. 45:2277–2287.)

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to the N-terminus of Mitochondrial antiviral-signaling protein.
Epitope	N-terminus
Host	Rabbit
Specificity	This antibody recognizes MAVS at the N-terminus. Splicing variants observed at ~33, 55, 60, and 65 kDa. (Lad, S., et al. (2008). Molecular Immunology 45:2277–2287.)
Species Reactivity	Human
Species Reactivity Note	Demonstrated to react with human. Other homologies: Chimpanzee (99% sequence homology). Rhesus macaque sequence homology). Rat (46% sequence homology). Mouse (44% sequence homology).
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_065797
Entrez Gene Summary	Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 (MIM 603029) or by the cytoplasmic RNA helicases MDA5 (MIM 606951) and RIGI (ROBO3; MIM 608630). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK; MIM 600664) and IKKB (IKBKB; MIM 603258), which activate NFKB (see MIM 164011), or TBK1 (MIM 604834) and IKKE (IKBKE; MIM 605048), which activate IRF3 (MIM 603734). Activated IRF3 and NFKB induce transcription of IFNB (IFNB1; MIM 147640). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1; MIM 607601). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005 [PubMed 16239922]).[supplied by OMIM].
Gene Symbol	MAVS VISA IPS-1 IPS1
Purification Method	Affinity Purfied
UniProt Number	Q7Z434
UniProt Summary	FUNCTION: Required for innate immune defense against viruses. Acts downstream of DDX58 and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis. SUBUNIT STRUCTURE: Interacts with DDX58, IFIH1, TRAF2 and TRAF6. May interact with IRF3, FADD, RIPK1, IKBKE, CHUK and IKBKB. Does not interact with TBK1. Interacts with and is cleaved by HCV and hepatitis GB virus B NS3/4A proteases. Interacts with and is cleaved by HHAV protein 3ABC. Interacts with NLRX1. Interaction with NLRX1 requires the CARD domain. Interacts with PSMA7. Interacts with TRAFD1 By similarity. Interacts (via C-terminus) with PCBP2 in a complex containing MAVS, PCBP2 and ITCH. Interacts with CYLD. SUBCELLULAR LOCATION: Mitochondrion outer membrane TISSUE SPECIFICTY: Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes. DOMAIN: Both CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58 and IFIH1. PTM: Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH; ITCH-dependent polyubiquitination is mediated by the interaction with PCBP2 and leads to MAVS proteosomal degradation. MISCELLANEOUS: Cleavage by HCV protease complex leads to inactivation. SEQUENCE SIMILARITIES: Contains 1 CARD domain. SEQUENCE CAUTION: The sequence BAA86585.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14684.1 differs from that shown. Reason: Frameshift at position 333.
Molecular Weight	~35, 55 and 65 kDa observed. Splicing variants were observed at ~35, 55 and 65 kDa. The additional band at ~60 kDa is annother potential splicing variant. (Lad, S., et al. (2008). Molecular Immunology. 45:2277–2287.)

Biological Information

Immunogen

GST-tagged recombinant protein corresponding to the N-terminus of Mitochondrial antiviral-signaling protein.

Epitope

N-terminus

Host

Rabbit

Specificity

This antibody recognizes MAVS at the N-terminus. Splicing variants observed at ~33, 55, 60, and 65 kDa. (Lad, S., et al. (2008). Molecular Immunology 45:2277–2287.)

Species Reactivity

Human

Species Reactivity Note

Demonstrated to react with human.
Other homologies: Chimpanzee (99% sequence homology). Rhesus macaque sequence homology). Rat (46% sequence homology). Mouse (44% sequence homology).

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_065797

Entrez Gene Summary

Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 (MIM 603029) or by the cytoplasmic RNA helicases MDA5 (MIM 606951) and RIGI (ROBO3; MIM 608630). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK; MIM 600664) and IKKB (IKBKB; MIM 603258), which activate NFKB (see MIM 164011), or TBK1 (MIM 604834) and IKKE (IKBKE; MIM 605048), which activate IRF3 (MIM 603734). Activated IRF3 and NFKB induce transcription of IFNB (IFNB1; MIM 147640). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1; MIM 607601). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005 [PubMed 16239922]).[supplied by OMIM].

Gene Symbol

MAVS
VISA
IPS-1
IPS1

Purification Method

Affinity Purfied

UniProt Number

Q7Z434

UniProt Summary

FUNCTION: Required for innate immune defense against viruses. Acts downstream of DDX58 and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis.

SUBUNIT STRUCTURE: Interacts with DDX58, IFIH1, TRAF2 and TRAF6. May interact with IRF3, FADD, RIPK1, IKBKE, CHUK and IKBKB. Does not interact with TBK1. Interacts with and is cleaved by HCV and hepatitis GB virus B NS3/4A proteases. Interacts with and is cleaved by HHAV protein 3ABC. Interacts with NLRX1. Interaction with NLRX1 requires the CARD domain. Interacts with PSMA7. Interacts with TRAFD1 By similarity. Interacts (via C-terminus) with PCBP2 in a complex containing MAVS, PCBP2 and ITCH. Interacts with CYLD. SUBCELLULAR LOCATION: Mitochondrion outer membrane

TISSUE SPECIFICTY: Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes.

DOMAIN: Both CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58 and IFIH1.

PTM: Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH; ITCH-dependent polyubiquitination is mediated by the interaction with PCBP2 and leads to MAVS proteosomal degradation.

MISCELLANEOUS: Cleavage by HCV protease complex leads to inactivation.

SEQUENCE SIMILARITIES: Contains 1 CARD domain.

SEQUENCE CAUTION: The sequence BAA86585.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB14684.1 differs from that shown. Reason: Frameshift at position 333.

Molecular Weight

~35, 55 and 65 kDa observed. Splicing variants were observed at ~35, 55 and 65 kDa. The additional band at ~60 kDa is annother potential splicing variant. (Lad, S., et al. (2008). Molecular Immunology. 45:2277–2287.)

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in A549 cell lysate. Western Blot Analysis: 1:1,000 dilution from a previous lot detected MAVS/Cardif on 10 µg of A549 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in A549 cell lysate.

Western Blot Analysis: 1:1,000 dilution from a previous lot detected MAVS/Cardif on 10 µg of A549 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-MAVS/Cardif, N-terminus

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