Anti-SRp30a (SF2) (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB, ICC	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

WB, ICC

Purified

Polyclonal Antibody

Description
Catalogue Number	06-1363
Description	Anti-SRp30a (SF2) Antibody
Alternate Names	serine/arginine-rich splicing factor 1 pre-mRNA-splicing factor SF2, P33 subunit SR splicing factor 1 splicing factor 2 splicing factor, arginine/serine-rich 1 Alternative-splicing factor 1
Background Information	SRp30a, also known as SF2, ASF-1 (alternative splicing factor 1), is a member of the highly conserved family of SR proteins, a group of serine/arginine-rich domain-containing proteins that play a critical role in regulated and constitutive splicing of precursor mRNA. The SR family of proteins is characterized as having one to two RNA recognition motifs located in the n-terminal sequence, and an RS domain within the C-terminus that is rich in serine/arginine dipeptide repeats. ASF-1 has an inhibitory role in exon skipping, in order to maintain splicing accuracy and to monitor alternative splicing. Studies have shown that ASF-1 plays a role in the regulation of caspase-9 alternative splicing as well as being a critical component for ceramide to function as a mediator of alternative splicing for the same caspase.

Description

Catalogue Number

06-1363

Description

Anti-SRp30a (SF2) Antibody

Alternate Names

serine/arginine-rich splicing factor 1
pre-mRNA-splicing factor SF2, P33 subunit
SR splicing factor 1
splicing factor 2
splicing factor, arginine/serine-rich 1
Alternative-splicing factor 1

Background Information

SRp30a, also known as SF2, ASF-1 (alternative splicing factor 1), is a member of the highly conserved family of SR proteins, a group of serine/arginine-rich domain-containing proteins that play a critical role in regulated and constitutive splicing of precursor mRNA. The SR family of proteins is characterized as having one to two RNA recognition motifs located in the n-terminal sequence, and an RS domain within the C-terminus that is rich in serine/arginine dipeptide repeats. ASF-1 has an inhibitory role in exon skipping, in order to maintain splicing accuracy and to monitor alternative splicing. Studies have shown that ASF-1 plays a role in the regulation of caspase-9 alternative splicing as well as being a critical component for ceramide to function as a mediator of alternative splicing for the same caspase.

Product Information
Format	Purified
Control	NIH/3T3 cell lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

NIH/3T3 cell lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Use Anti-SRp30a (SF2) Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect SRp30a (SF2) also known as serine/arginine-rich splicing factor 1, pre-mRNA-splicing factor SF2- P33 subunit, SR splicing factor 1.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected SRp30a in NIH/3T3 cells.

Applications

Application

Use Anti-SRp30a (SF2) Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect SRp30a (SF2) also known as serine/arginine-rich splicing factor 1, pre-mRNA-splicing factor SF2- P33 subunit, SR splicing factor 1.

Key Applications

Western Blotting
Immunocytochemistry

Application Notes

Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected SRp30a in NIH/3T3 cells.

Biological Information
Immunogen	Linear peptide corresponding to human SRp30a.
Host	Rabbit
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001071634
Entrez Gene Summary	This gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 13. [provided by RefSeq].
Gene Symbol	ASF-1 SF2 ASF SFRS1 SF2p33 SRp30a SRSF1 SF2P333
Purification Method	Protein A
UniProt Number	Q07955
UniProt Summary	FUNCTION: Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. SUBUNIT STRUCTURE: Consists of two polypeptides of p32 and p33. In vitro, self-associates and binds SRSF2, SNRNP70 and U2AF1 but not U2AF2. Binds SFRS12. Interacts with SAFB/SAFB1. Interacts with PSIP1/LEDGF. Interacts with SRPK1. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SRSF1/SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Interacts with RSRC1 (via Arg/Ser-rich domain). Interacts with ZRSR2/U2AF1-RS2. SUBCELLULAR LOCATION: Cytoplasm. Nucleus speckle. Note: In nuclear speckles. Shuttles between the nucleus and the cytoplasm. DOMAIN: Both the RS domain and an RRM domain are required for nucleocytoplasmic shuttling. PTM: Extensively phosphorylated on serine residues in the RS domain. This may regulate nucleocytoplasmic shuttling. Arg-97 is dimethylated, probably to asymmetric dimethylarginine. SEQUENCE SIMILARITIES: Belongs to the splicing factor SR family. Contains 2 RRM (RNA recognition motif) domains.
Molecular Weight	~40 kDa observed. An uncharacterized band at ~ 50 kDa has been observed in some lysates.

Biological Information

Immunogen

Linear peptide corresponding to human SRp30a.

Host

Rabbit

Species Reactivity

Human
Mouse
Rat

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_001071634

Entrez Gene Summary

This gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 13. [provided by RefSeq].

Gene Symbol

ASF-1
SF2
ASF
SFRS1
SF2p33
SRp30a
SRSF1
SF2P333

Purification Method

Protein A

UniProt Number

Q07955

UniProt Summary

FUNCTION: Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors.

SUBUNIT STRUCTURE: Consists of two polypeptides of p32 and p33. In vitro, self-associates and binds SRSF2, SNRNP70 and U2AF1 but not U2AF2. Binds SFRS12. Interacts with SAFB/SAFB1. Interacts with PSIP1/LEDGF. Interacts with SRPK1. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SRSF1/SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Interacts with RSRC1 (via Arg/Ser-rich domain). Interacts with ZRSR2/U2AF1-RS2.

SUBCELLULAR LOCATION: Cytoplasm. Nucleus speckle. Note: In nuclear speckles. Shuttles between the nucleus and the cytoplasm.

DOMAIN: Both the RS domain and an RRM domain are required for nucleocytoplasmic shuttling.

PTM: Extensively phosphorylated on serine residues in the RS domain. This may regulate nucleocytoplasmic shuttling.

Arg-97 is dimethylated, probably to asymmetric dimethylarginine.

SEQUENCE SIMILARITIES: Belongs to the splicing factor SR family.

Contains 2 RRM (RNA recognition motif) domains.

Molecular Weight

~40 kDa observed. An uncharacterized band at ~ 50 kDa has been observed in some lysates.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in NIH/3T3 cell lysate. Western Blot Analysis: 1:500 dilution of this antibody detected SRp30a on 10 µg of NIH/3T3 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected SRp30a on 10 µg of NIH/3T3 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-SRp30a (SF2) (rabbit polyclonal)

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