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Anti-Fox1, clone D8F8 (mouse monoclonal)

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货号：MABE159

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M WB M Purified Monoclonal Antibody
Description
Catalogue Number MABE159
Description Anti-Fox1 Antibody, clone D8F8
Alternate Names
  • hexaribonucleotide binding protein 1
  • Ataxin-2-binding protein 1
  • ataxin 2-binding protein 1
  • Hexaribonucleotide-binding protein 1
  • fox-1 homolog A
Background Information Fox1, also commonly referred to as Ataxin-2-binding protein 1, is an RNA binding protein involved in the regulation of tissue-specific alternative splicing. This protein contains a highly conserved RRM (RNA recognition motif) and associates with 5'-UGCAUGU-3' elements. This protein has been demonstrated to regulate alternative splicing of tissue specific exons and differentially spliced exons during erythropoiesis. Its alternative name, Ataxin-2-binding protein, is a result of its association with the C terminus of ataxin 2 which may contribute to the pathology of the neurogenerative disease spinocerebellar ataxia type 2 (SCA2).
References
Product Information
Format Purified
Control
  • Mouse heart tissue lysate
Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-Fox1 Antibody, clone D8F8 is a Mouse Monoclonal Antibody for detection of Fox1 also known as hexaribonucleotide binding protein 1, Ataxin-2-binding protein 1 & has been validated in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen Recombinant protein corresponding to mouse Fox1.
Clone D8F8
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG1κ
Species Reactivity
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FOX1
  • A2BP1
  • A2BP
Purification Method Protein G
UniProt Number
UniProt Summary FUNCTION: RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.

SUBUNIT STRUCTURE: Binds to the C-terminal of ATXN2.

SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Predominantly nuclear.

TISSUE SPECIFICTY: Detected in brain (at protein level). Detected in heart, brain, neurons, skeletal muscle and embryo.

SEQUENCE SIMILARITIES: Contains 1 RRM (RNA recognition motif) domain.
Molecular Weight ~55 kDa observed. Uncharacterized bands potentially due to alternative splicing have been observed at ~33 and 37 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in mouse heart tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Fox1 on 10 µg of mouse heart tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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