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Ms X CASK

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货号：MAB5230

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R, H WB, IH(P) M Purified Monoclonal Antibody
Description
Catalogue Number MABN1547
Replaces MAB5230
Description Anti-CASK Antibody, clone 2G5.1
Alternate Names
  • Peripheral plasma membrane protein CASK
  • hCASK
  • Calcium/calmodulin-dependent serine protein kinase
  • Protein lin-2 homolog
  • CASK
Background Information Peripheral plasma membrane protein CASK (EC 2.7.11.1; UniProt O14936; also known as Calcium/calmodulin-dependent serine protein kinase, Membrane-associated guanylate kinase, hCASK, Protein lin-2 homolog, Trinucleotide repeat containing 8) is encoded by the CASK (also known as CAGH39, CAMGUK, CMG, FGS4, LIN2, MICPCH, MRXSNA, TNRC8) gene (Gene ID 8573) in human. CASK is a membrane-associated guanylate kinase (MAGUK) family member involved in the regulation of cytoskeletal remodeling and cell migration. It consists of a Ca2+-calmodulin kinase, a PDZ, an SH3, and a guanylate kinase domain. CASK localizes at cell–cell adhesion sites, including synaptic junctions, epithelial junctions, and tight junctions, where it is reported to associate with other cell adhesion molecules, such as junctional adhesion molecule (JAM), cadherins, catenins, and syndecan-2. CASK and syndecan-2 expressions are upregulated in colorectal cancer and their upregulation significantly correlates with advanced tumor stage, lymphatic invasion, lymph node metastasis, vascular invasion, liver metastasis, and unresectable metastatic CRC.
References
Product Information
Format Purified
Presentation Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This Anti-CASK Antibody, clone 2G5.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of CASK.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application Notes Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CASK in human kidney cortex tissue.
Biological Information
Immunogen GST-tagged recombinant protein corresponding to an internal fragment within the C-terminal half of human CASK.
Epitope Internal (C-terminal half)
Clone 2G5.1
Concentration Please refer to lot specific datasheet.
Host Mouse
Isotype IgG2aκ
Species Reactivity
  • Rat
  • Human
Species Reactivity Note Rat, Human. Predicted to react with Mouse based on 100% sequence homology.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CASK
  • LIN2
Purification Method Protein G Purified
UniProt Number
Molecular Weight ~115 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CASK in 10 µg of rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 μg
Transport Information
Supplemental Information
Specifications

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