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Anti-Mn-SOD

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货号：06-984

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, R, M, B IHC, IH(P), WB Rb Purified Polyclonal Antibody
Description
Catalogue Number 06-984
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-Mn-SOD Antibody
Alternate Names
  • Mn superoxide dismutase
  • indophenoloxidase B
  • manganese superoxide dismutase
  • manganese-containing superoxide dismutase
  • mangano-superoxide dismutase
  • superoxide dismutase 2, mitochondrial
Background Information Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). The manganese containing 80 kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain.
References
Product Information
Format Purified
Control
  • L6 cell lysate, rat brain tissue extract.
Presentation Purified rabbit polyclonal in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
Applications
Application Anti-Mn-SOD Antibody is an antibody against Mn-SOD for use in IH, IH(P) & WB.
Key Applications
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application Notes Immunohistochemistry (Paraffin):
10 μg/mL of a previous lot detected MnSOD in paraffin embedded rat brain tissue sections.
Biological Information
Immunogen Recombinant human MnSOD (manganous superoxide dismutase).
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes MnSOD, Mr 24 kDa.
Isotype IgG
Species Reactivity
  • Human
  • Rat
  • Mouse
  • Bovine
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Gene Symbol
  • SOD2
  • Mn-SOD
  • MNSOD
  • MnSOD
  • IPO-B
Non-Reactive Species
  • Canine
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: P04179 # Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
COFACTOR: Binds 1 manganese ion per subunit.
SIZE: 222 amino acids; 24722 Da
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Mitochondrion matrix.SIMILARITY:SwissProt: P04179 ## Belongs to the iron/manganese superoxide dismutase family.
Molecular Weight ~24 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by western blot on RIPA lysates of MCF-7, A431 or rat L6 RIPA cells.

Western Blot Analysis:
0.5-2 μg/mL of this antibody detected MnSOD in RIPA lysates of rat L6 RIPA cells. 1-4 μg/mL of a previous lot detected MnSOD in RIPA lysates of human MCF-7, A431 and rat L6 RIPA cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2 to 8ºC from date of receipt.
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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