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The Jurkat Apoptotic Lysate Set I is designed to be used as a western blot positive control for detecting antigens expressed during apoptosis. Jurkat cells in log-phase growth were either left untreated or treated with 4 µM camptothecin for 4 hours to induce apoptosis. The cells were collected, lysed in lysis buffer [1% SDS, 1 mM sodium orthovanadate, 10 mM Tris (pH 8.0)], and aliquoted in SDS sample buffer.
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