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Calcein AM 1mg

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0.00
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号:564061

牌:BD

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Brand
BD Pharmingen™
Application
Bioimaging, Flow cytometry (Tested During Development) 
Regulatory Status
RUO

Regulatory Status Legend

RUO
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
RUO (GMP)
For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices.
GPR
General Purpose Reagent
IVD
For In Vitro Diagnostic Use.
ASR
Analyte Specific Reagent. Analytical and performance characteristics are not established.

Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.

Description

BD Pharmingen™ Calcein AM is used for labeling live cells that can be detected and further analyzed by flow cytometry or fluorescence imaging. The enhanced hydrophobicity of the acetomethoxy (AM) derivative of Calcein allows this dye to readily enter viable cells. Once inside, intracellular esterases cleave the AM groups off of the non-fluorescent BD Pharmingen™ Calcein AM, trapping fluorescent Calcein within the cell. Since dead cells lack esterase activity, only viable cells are labeled. Calcein AM is optimally excited at the 495 nm wavelength of light and emits maximally at 515 nm.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  3. Accutase is a registered trademark of Innovative Cell Technologies, Inc.
  4. All other brands are trademarks of their respective owners.
  5. Cy is a trademark of GE Healthcare.
  6. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
  7. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.

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