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Calcein Blue AM 1mg

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0.00
价:
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号:564060

牌:BD

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Brand
BD Pharmingen™
Application
Flow cytometry (Tested During Development) 
Regulatory Status
RUO

Regulatory Status Legend

RUO
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
RUO (GMP)
For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices.
GPR
General Purpose Reagent
IVD
For In Vitro Diagnostic Use.
ASR
Analyte Specific Reagent. Analytical and performance characteristics are not established.

Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.

Description

BD Pharmingen™ Calcein Blue AM is used for labeling live cells that can be detected and further analyzed by flow cytometry. The enhanced hydrophobicity provided by the acetomethoxy (AM) group allows this dye to readily enter viable cells. Once inside, intracellular esterases cleave the AM groups off of the weakly fluorescent Calcein Blue AM, trapping fluorescent Calcein Blue within the cell. Since dead cells lack esterase activity, only viable cells are labeled. Calcein Blue is optimally excited at the 360 nm wavelength of light that can be provided by an ultraviolet laser and emits maximally at 445 nm. This dye can also be excited by the violet laser.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  3. Accutase is a registered trademark of Innovative Cell Technologies, Inc.
  4. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
  5. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.

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