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Store undiluted at 4°C.
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Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
The Annexin V Binding Buffer, 10X concentrate has been formulated for use in flow cytometric assays utilizing Annexin V. The Annexin V Binding Buffer is a 10X concentrate composed of a 0.2 µm sterile filtered 0.1M Hepes (pH 7.4), 1.4M NaCl, and 25 mM CaCl2 solution. Prior to staining cells, an appropriate quantity of a 1X working solution should be made by diluting the 10X concentrate 1:10 with distilled water. Any remaining 1X solution at the end of the experiment should be discarded.
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