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CFSE 1mg

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0.00
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号:565082

牌:BD

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Brand
BD Horizon™
Application
Intracellular staining (flow cytometry) (Routinely Tested) 
Regulatory Status
RUO

Regulatory Status Legend

RUO
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
RUO (GMP)
For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices.
GPR
General Purpose Reagent
IVD
For In Vitro Diagnostic Use.
ASR
Analyte Specific Reagent. Analytical and performance characteristics are not established.

Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.

Description

BD Horizon™ CFSE (carboxyfluorescein diacetate succinimidyl ester) is a blue laser excitable dye that can be used for flow cytometric monitoring of cell divisions. The non-fluorescent dye passively diffuses across cell membranes and is cleaved by intracellular esterases within viable cells. The cleaved dye becomes highly fluorescent and covalently binds to protein amine groups within the cells via its succinimidyl ester group. Nonviable cells remain nonfluorescent. As viable cells divide, CFSE dye is distributed uniformly between daughter cells; each daughter cell retains approximately half of the CFSE fluorescence intensity of its parent cell. The covalently bound dye is well retained within the cells allowing for applications involving live or aldehyde-fixed and permeabilized cells.

BD Horizon™ CFSE has an excitation maximum of 490 nm and an emission maximum of 520 nm.

Preparation and Storage

Store at -20°C, protected from exposure to light.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  3. Cy is a trademark of GE Healthcare.
  4. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
  5. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.

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