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BD Horizon™ CFSE (carboxyfluorescein diacetate succinimidyl ester) is a blue laser excitable dye that can be used for flow cytometric monitoring of cell divisions. The non-fluorescent dye passively diffuses across cell membranes and is cleaved by intracellular esterases within viable cells. The cleaved dye becomes highly fluorescent and covalently binds to protein amine groups within the cells via its succinimidyl ester group. Nonviable cells remain nonfluorescent. As viable cells divide, CFSE dye is distributed uniformly between daughter cells; each daughter cell retains approximately half of the CFSE fluorescence intensity of its parent cell. The covalently bound dye is well retained within the cells allowing for applications involving live or aldehyde-fixed and permeabilized cells.
BD Horizon™ CFSE has an excitation maximum of 490 nm and an emission maximum of 520 nm.
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