miRNA was isolated from 200 µl plasma from 3 different samples using the miRNeasy Serum/Plasma Advanced Kit (light blue bars) or the miRNeasy Serum/Plasma Kit (dark blue bars). Five microliters of recovered RNA, in a total volume of 25 µl, was used for cDNA synthesis using the miScript system, followed by PCR detection. Both kits show good recovery of miR16.

miRNA was isolated from 200 µl plasma from 20 different samples using the miRNeasy Serum/Plasma Advanced Kit. Five microliters of recovered RNA, in a total volume of 25 µl, was used for cDNA synthesis using the miScript system, followed by PCR detection of 3 different miRNAs found to be primarily present inside or outside of extracellular vesicle, respectively. The miRNeasy Serum/Plasma Advanced Kit can detect miRNAs both inside and outside of vesicles.

Part of this result have been generated within the CANCER ID project, funded by the Innovative Medicines Initiative 2 Joint Undertaking (JU) under grant agreement No 115749. The JU receives support from the European Union’s Horizon 2020 research and innovation program and EFPIA.

The miRNeasy Serum/Plasma Advanced Kit shows comparable or better performance to kits from other suppliers. miRNA (A) or mRNA (B) was isolated from 200 µl plasma from 2 different samples using the miRNeasy Serum/Plasma Advanced Kit or kits from other suppliers. Depending on the elution volume, an equal amount of the recovered RNA was applied in a total volume of (A) 25 µl for cDNA synthesis using the miScript system, followed by PCR detection of miR122a and miR150 miRNA, respectively, or (B) applied in a total volume of 20 µl for RT-PCR using the QuantiFast SYBR Green Kit and detection of mRNA-RPL12. The miRNeasy Serum/Plasma Advanced Kit shows comparable or better performance compared to kits from other suppliers for both miRNAs.