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Qiagen 凯杰 Biosharp Omega

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miRCURY LNA RT Kit

价:
6780.00
价:
¥5424.00

号:339340

牌:Qiagen 凯杰

账期 货到付款

EA (预计3-5工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

客服 郭恒 蔡玉坤 曾宪飞 技术咨询

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For optimal first-strand cDNA synthesis with the miRCURY LNA miRNA PCR System
  • Optimized for use with miRCURY LNA miRNA PCR Assays and Panels
  • Fast, easy and reliable first-strand synthesis
  • Includes RNA spike-in template for monitoring performance

The miRCURY LNA RT Kit enables fast and convenient miRNA polyadenylation and reverse transcription in a single reaction step and has been designed for use with the miRCURY LNA miRNA PCR System. Using one universal RT reaction as the template for subsequent PCR amplifications greatly simplifies the procedure compared with systems that require miRNA-specific first-strand synthesis, adding convenience and saving you time.

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Accurate quantitation from down to 1 pg total RNA starting material.
Data from serial dilutions of human AM6000 total RNA are shown. All miRNA assays exhibit linear readout with correlation coefficients R 2 > 0.99.
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miRCURY LNA miRNA PCR System at a glance.
The miRCURY LNA miRNA PCR System uses one single cDNA synthesis reaction for all amplifications, reducing pipetting and saving time and sample. Two LNA-enhanced miRNA-specific qPCR primers enable highly specific and sensitive amplification and single nucleotide discrimination. The fast and easy workflow takes only 3 hours with minimal hands-on steps.


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The benefit of a universal reverse transcription step.
One universal RT reaction per RNA sample saves not only reagents and precious samples, but also your time.
Performance
Unmatched sensitivity
Universal reverse transcription combined with LNA-enhanced and T m-normalized primers enables accurate and reliable quantification of individual miRNAs from as little as 1 pg total RNA (see figure Accurate quantitation from down to 1 pg total RNA starting material). With exceptional sensitivity and extremely low background, you can accurately quantify very low levels of miRNA without pre-amplification. This makes the miRCURY LNA miRNA PCR System suitable for all types of samples, especially those with low RNA content, such as serum, plasma and other biofluids.
Principle
High-performance reagents for quantitative, real-time RT-PCR

miRCURY LNA RT Kit
This kit includes all the necessary reagents to perform fast and convenient miRNA polyadenylation and reverse transcription in a single reaction step. Using a single, universal RT reaction as the template for subsequent PCR amplifications greatly simplifies the procedure compared to systems that require miRNA-specific, first-strand synthesis (see figure The benefit of a universal reverse transcription step). The UniSp6 RNA spike-in can be used with the control assay – UniSp6 miRCURY LNA miRNA PCR Assay (cat. no. YP00203954) – as a reverse transcription positive control and/or as an inter-plate calibrator in our miRNA PCR panels.

miRCURY LNA SYBR Green PCR Kit
This kit contains the high-performance miRCURY SYBR Green Master Mix for quantitative real-time PCR amplification. The detection is based on SYBR Green, which allows quality control of the resulting PCR amplicon by melting curve analysis. LNA-enhanced primer sets require specific PCR reaction conditions for optimal performance. The kit also includes a control primer set designed for amplification of the synthetic RNA spike-in provided in the miRCURY LNA RT Kit. Refer to the miRCURY LNA RT Kit Handbook for details. The miRCURY LNA miRNA PCR Assays and Panels have been validated using the miRCURY SYBR Green Master Mix; use of other reagents for qPCR will affect the results.
Procedure
The miRCURY LNA miRNA PCR Assay system is an miRNA-specific, LNA-based system designed for sensitive and accurate detection of miRNA by quantitative real-time PCR using SYBR Green. The first step of the procedure is universal reverse transcription, followed by real-time PCR amplification with LNA-enhanced primers (see figure miRCURY LNA miRNA PCR System at a glance).
Applications
The miRCURY LNA RT Kit is used as part of the miRCURY PCR System for:
  • Mature miRNA quantification and profiling
  • Mature miRNA and precursor miRNA detection in parallel
  • snoRNA RNA detection

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