Aliquots of 200 mg of three different human stool specimens were processed on the QIAcube HT using the QIAamp 96 PowerFecal QIAcube HT Kit, on the QIAcube using the QIAamp DNA Stool Mini Kit and QIAamp Fast DNA Stool Mini Kit, or manually using the two DNA Stool kits. Real-time PCR of β-Actin was carried out on the Rotor-Gene Q using the QuantiFast Probe PCR Kit with 2 µl eluate in a 25 µl reaction. Human DNA purified either using different instrument platforms or manually from all the three specimens showed similar performance in the assay.
Aliquots of 200 mg of three different human stool specimens were processed on the QIAcube HT using the QIAamp 96 PowerFecal QIAcube HT Kit, on the QIAcube using the QIAamp DNA Stool Mini Kit and QIAamp Fast DNA Stool Mini Kit, or manually using the two DNA Stool kits. Real-time PCR of generic 16S rRNA gene sequence was carried out on the Rotor-Gene Q using the QuantiFast Probe PCR Kit with 1 µl eluate in a 20 µl reaction. DNA extracted either using different instrument platforms or manually from all the three specimens showed similar performance in the assay.
Aliquots of 200 mg of three different human stool specimens were processed on the QIAcube HT using the QIAamp 96 PowerFecal QIAcube HT Kit, on the QIAcube using the QIAamp DNA Stool Mini Kit and QIAamp Fast DNA Stool Mini Kit, or manually using the two DNA Stool kits. Real-time PCR of 16S rRNA gene of Corynebacterium glutamicum was carried out on the Rotor-Gene Q using the QuantiFast Probe PCR Kit with 1 µl eluate in a 20 µl reaction. DNA extracted either using different instrument platforms or manually from all the three specimens showed similar performance in the assay.
Aliquots of 200 mg of three different human stool specimens were processed on the QIAcube HT using the QIAamp 96 PowerFecal QIAcube HT Kit, on the QIAcube using the QIAamp DNA Stool Mini Kit and QIAamp Fast DNA Stool Mini Kit, or manually using the two DNA Stool kits. Real-time PCR was carried out on the Rotor-Gene Q using the QuantiTect Virus PCR Kit with 2 µl and 10 µl eluates respectively, from each sample in 20 µl reactions containing an internal control. C T values were compared to a standard containing no sample but only internal control (dark blue bar). All the three samples either extracted on different instruments or manually showed similar performance compared to the standard, regardless of the volume of eluate used, indicating no PCR inhibition.
Aliquots of 200 mg stool sample from different animals were processed on the QIAcube HT instrument (blue bars) using the QIAamp 96 PowerFecal QIAcube HT Kit, or manually using the QIAamp Fast DNA Stool Mini Kit (pink bars). Real-time PCR of 16S rRNA gene of Corynebacterium glutamicum was carried out on the Rotor-Gene Q using the QuantiFast Probe PCR Kit with 1 µl eluate in a 20 µl reaction. DNA extracted either manually or on the QIAcube HT instrument showed similar performance for all the stool samples.
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