For the isolation of microbial DNA from stool gut samples
Efficient lysis of bacteria fungi from stool gut samples
Up to 20-fold more DNA compared to alternative methods
Yield higher alpha diversity in sequencing compared to other methods
Recovery of inhibitor-free DNA for direct use in next-generation sequencing applications
Automate workflows on the QIAcube
Extracting microbial DNA from stool gut samples can be challenging. Researchers will isolate higher yields of pure microbial DNA from stool gut samples more efficiently with our new QIAamp PowerFecal Pro DNA Kit.
The QIAamp PowerFecal Pro DNA Kit builds improves on our original PowerFecal technology utilizing a novel bead tube optimized chemistry for more efficient lysis of bacteria fungi. Inhibitor removal has been streamlined, allowing researchers to eliminate challenging inhibitors from stool gut samples faster. This results in total DNA that reveals higher alpha diversity through observed operational taxonomic units (OTUs) in downstream testing.
Higher yields of DNA with the QIAamp PowerFecal Pro DNA Kit.
DNA was isolated from stool samples (200 mg) from three donors using commercially available sample preparation solutions the new QIAamp PowerFecal Pro DNA Kit compared. Yields were measured by fluorometric quantification (Qubit™).
The new QIAamp PowerFecal Pro DNA Kit enables the isolation of larger amounts of high-quality DNA.
A DNA from duplicate 1.8 ml C. albicans cultures was isolated with the QIAamp PowerFecal DNA (A) the QIAamp PowerFecal Pro DNA (B) Kits. B, C DNA from stool samples (200 mg) from three donors was prepared with the first-generation QIAamp PowerFecal DNA Kit B the new QIAamp PowerFecal Pro DNA Kit C. DNA was analyzed via gel electrophoresis.
Highly pure DNA isolated using the new QIAamp PowerFecal Pro DNA Kit.
Stool samples (200 mg) were prepared using commercially available sample preparation solutions the new QIAamp PowerFecal Pro DNA Kit compared.
DNA purity was measured via UV spectrophotometry the comparisons of A 260/A 280A A 260/A 230B ratios are shown..
Greater total number of bacterial OTUs isolated with the new QIAamp PowerFecal Pro DNA Kit.
DNA prepared from stool samples was isolated with different methods enriched. Analysis of the 16S rRNA genes was done using the QIAseq ® 1-Step Amplicon Kit data analysis was done using the Microbial Genomics Pro DNA Suite (CLC workbench). Alpha diversity was determined by total number of bacterial OTUs.
Performance
Using improved technologies, the new QIAamp PowerFecal Pro Kit allows researchers to obtain higher yields of DNA from stool gut samples compared with alternative methods (See: ‘ Higher yields of DNA with the QIAamp PowerFecal Pro DNA Kit.’).
Improved yields of higher quality DNA are also achieved when comparing the QIAamp PowerFecal Pro DNA Kit to the first generation QIAamp PowerFecal DNA Kit when running stool fungal samples (See: ‘ The new QIAamp PowerFecal Pro DNA Kit enables the isolation of larger amounts of high quality DNA.’).
Principle
The streamlined Inhibitor Removal Technology ® (IRT) used by the new QIAamp PowerFecal Pro DNA Kit is highly efficient at removing inhibitors while still decreasing sample processing time. The QIAamp PowerFecal Pro DNA Kits also showed the highest A 260/A 280 ratios, near 1.8, of any other commercially available kit, indicating the absence of inhibitors. Less variability was also observed between the samples processed with QIAamp PowerFecal Pro DNA Kits (See: ‘ Highly pure DNA isolated using the new QIAamp PowerFecal Pro DNA Kit.’). Researchers using the QIAamp PowerFecal Pro DNA Kit can identify a greater bacterial diversity with increased OTUs from stool samples compared with the first-generation QIAamp PowerFecal Kit other commercially available kits (See: ‘ Greater total number of bacterial OTUs isolated with the new QIAamp PowerFecal Pro DNA Kit.’).
Procedure
Samples processed with the QIAamp PowerFecal Pro DNA Kit are added to a bead beating tube. Rapid thorough homogenization occurs using mechanical chemical methods. Once cells are lysed, our patented IRT is used to remove inhibitors. Total genomic DNA is captured on a silica membrane in a spin-column format. DNA is then washed eluted, ready for downstream applications.
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