TESTED APPLICATIONS | DILUTION |
---|---|
Immunocytochemistry (ICC) | 1:500 |
Immunofluorescence (IF) | 1:500 |
Immunohistochemistry (IHC) | 1:500 |
Immunoprecipitation (IP) | 8 µg |
Western Blot (WB) | 1:300-1:500 |
Species reactivity | Human, Mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Recombinant human Ago2 protein (GST fusion protein containing amino acids 47-879 of hAgo2). |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 0.0024 mg/mL |
Purification | Protein A |
Storage buffer | PBS, pH 7.4 |
Contains | 0.05% sodium azide |
Storage conditions | -20°C or -80°C if preferred |
RRID | AB_2576596 |
AGO2 is a member of the Argonaute family. These proteins are involved in short-interfering-RNA-mediated gene silencing. This process is mediated by the RISC (RNA induced silencing complex), a multicomponent nuclease that is able to degrade double stranded RNAs. The mRNA degradation is triggered by the association of specific siRNAs with their homologous mRNAs. IEF2C2 has been shown to be essential for embryonic development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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