Bak BH3 10mg

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Qiagen 凯杰 Biosharp Omega

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首页酶、辅酶及抑制剂MCE>

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市场价：: 5500.00

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商品介绍
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Bak BH3 是一种多肽，源于 Bak 蛋白的 BH3 结构域，在细胞中，能够抑制 Bcl-xL 的活性。

Description

Bak BH3 is derived from the BH3 domain of Bak, can antagonize the function of Bcl-xL in cells.

IC₅₀ & Target^[1]

Bcl-xL

In Vitro

Bak BH3 peptide antagonize the protective effects of microinjected Bcl-xL in α-Fas-treated HeLa cells, whereas a mutant Bak BH3 peptide that no longer binds Bcl-xL is inactive^[1].

Solvent & Solubility

In Vitro:

10 mM in DMSO

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.5797 mL	2.8987 mL	5.7974 mL
5 mM	0.1159 mL	0.5797 mL	1.1595 mL
10 mM	0.0580 mL	0.2899 mL	0.5797 mL

* Please refer to the solubility information to select the appropriate solvent.

References

[1]. Holinger EP, et al. Bak BH3 peptides antagonize Bcl-xL function and induce apoptosis through cytochrome c-independent activation of caspases. J Biol Chem. 1999 May 7;274(19):13298-304.

Cell Assay ^[1]	Cells are plated in complete DMEM in 96-well tissue culture plates at 4×10³/well. After 24 h, cells are washed with PBS and treated with peptides (50 μM) in SF-DMEM. Cell viability is determined by staining unfixed cells with calcein AM/ethidium homodimer, followed by microscopic analysis of cell staining and cellular morphology on a Nikon Diaphot 300 inverted microscope equipped with a fluorescence module. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Holinger EP, et al. Bak BH3 peptides antagonize Bcl-xL function and induce apoptosis through cytochrome c-independent activation of caspases. J Biol Chem. 1999 May 7;274(19):13298-304.

Cell Assay
^[1]

Cells are plated in complete DMEM in 96-well tissue culture plates at 4×10³/well. After 24 h, cells are washed with PBS and treated with peptides (50 μM) in SF-DMEM. Cell viability is determined by staining unfixed cells with calcein AM/ethidium homodimer, followed by microscopic analysis of cell staining and cellular morphology on a Nikon Diaphot 300 inverted microscope equipped with a fluorescence module.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Holinger EP, et al. Bak BH3 peptides antagonize Bcl-xL function and induce apoptosis through cytochrome c-independent activation of caspases. J Biol Chem. 1999 May 7;274(19):13298-304.

Molecular Weight	1724.90
Formula	C₇₂H₁₂₅N₂₅O₂₄
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Shipping	Room temperature in continental US; may vary elsewhere