BAM7 纯度:99.57% 10mg

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BAM7 纯度:99.57% 10mg

市场价：: 1083.00

促销价：: ¥1083.00

货号：HY-15341

品牌：MCE

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北京朝阳

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商品介绍
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BAM7 是一种直接的选择性 BAX 激活剂，IC₅₀ 为 3.3 μM。

Description

BAM7 is a direct and selective activator of proapoptotic BAX with an IC₅₀ of 3.3 μM.

IC₅₀ & Target^[1]

Bax

3.3 μM (IC₅₀)

In Vitro

BAM7 is selective for the BH3-binding site on BAX. BAM7 activates BAX and BAX-dependent cell death. Whereas treatment with BAX or BAM7 alone has no effect on the liposomes, the combination of BAM7 and BAX yields dose-responsive liposomal release of entrapped fluorophore. BAM7 dose- and time-responsively impairs the viability of Bak^-/- MEFs that exclusively express BAX but has no effect on Bak^-/- MEFs that contain BAK but lack BAX. In contrast, standard proapoptotic stimuli such as serum withdrawal, Staurosporine and Etoposide induces an equivalent apoptotic response in Bax^-/- and Bak^-/- MEFs. As further evidence of BAM7 specificity of action, (i) BAM7 does not affect the viability of Bax^-/- Bak^-/- MEFs; (ii) ANA-BAM16, which does not bind or activate BAX, has no effect on Bak^-/- MEFs; and (iii) BAM7 selectively induces cell death of Bax^-/- Bak^-/- MEFs reconstituted with wild-type BAX but not BAXK21E , which bears the mutation that abrogates BAM7 binding^[1].

Solvent & Solubility

In Vitro:

10 mM in DMSO

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4663 mL	12.3314 mL	24.6627 mL
5 mM	0.4933 mL	2.4663 mL	4.9325 mL
10 mM	0.2466 mL	1.2331 mL	2.4663 mL

* Please refer to the solubility information to select the appropriate solvent.

References

[1]. Gavathiotis E, et al. Direct and selective small-molecule activation of proapoptotic BAX. Nat Chem Biol. 2012 Jul;8(7):639-45.

Cell Assay ^[1]	MEF cells are maintained in DMEM high glucose supplemented with 10% (v/v) FBS, 100 U/mL Penicillin, 100 μg/mL Streptomycin, 2 mM L-glutamine, 50 mM HEPES, 0.1 mM MEM nonessential amino acids and 50 μM β-mercaptoethanol. MEFs (2.5×10³ cells per well) are seeded in 96-well opaque plates for 18-24 h and then incubated with serial dilutions of BAM7 (3.75, 5, 7.5, 10 and 15 μM), ANA-BAM16 or vehicle (0.15% (v/v) DMSO) in DMEM at 37°C in a final volume of 100 μL. Cell viability is assayed at 24 h by addition of CellTiter-Glo reagent, and luminescence is measured using a SpectraMax M5 microplate reader. Viability assays are performed in at least triplicate, and the data are normalized to vehicle-treated control wells^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Gavathiotis E, et al. Direct and selective small-molecule activation of proapoptotic BAX. Nat Chem Biol. 2012 Jul;8(7):639-45.

Cell Assay
^[1]

MEF cells are maintained in DMEM high glucose supplemented with 10% (v/v) FBS, 100 U/mL Penicillin, 100 μg/mL Streptomycin, 2 mM L-glutamine, 50 mM HEPES, 0.1 mM MEM nonessential amino acids and 50 μM β-mercaptoethanol. MEFs (2.5×10³ cells per well) are seeded in 96-well opaque plates for 18-24 h and then incubated with serial dilutions of BAM7 (3.75, 5, 7.5, 10 and 15 μM), ANA-BAM16 or vehicle (0.15% (v/v) DMSO) in DMEM at 37°C in a final volume of 100 μL. Cell viability is assayed at 24 h by addition of CellTiter-Glo reagent, and luminescence is measured using a SpectraMax M5 microplate reader. Viability assays are performed in at least triplicate, and the data are normalized to vehicle-treated control wells^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References