Bax inhibitor peptide V5 纯度:99.79% 10mg

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首页酶、辅酶及抑制剂MCE>

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Bax inhibitor peptide V5 纯度:99.79% 10mg

市场价：: 1400.00

促销价：: ¥1400.00

货号：HY-P0081

品牌：MCE

配送至：

北京朝阳

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支 (预计4-5工作日到货(以MCE官网货期为准))

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商品介绍
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Bax inhibitor peptide V5 是一种 Bax 诱导凋亡的抑制剂，主要用于癌症研究。

Description

Bax inhibitor peptide V5 is a Bax-mediated apoptosis inhibitor, used for cancer treatment.

IC₅₀ & Target^[1]

Bax

In Vitro

Bax inhibitor peptide V5 (0-50 μM) reduces cell death in STF-cMyc cells but not in SW620 or NCI-H23 cells. BIPV5 does not result in any significant effect on cell cycle arrest at the G2/M phase^[1]. V5 treatment upregulates expression of anti-apoptotic proteins Bcl-2 and XIAP by more than 3- and 11-fold and downregulates expression of apoptosis-inducing proteins Bax, Bad, and nuclear factor-κB-p65 by 10, 30, and nearly 50%, respectively^[2].

In Vivo

V5 (100 μM) significantly improves islet function following isolation and improves islet graft function following transplantation in mice model^[2].

Solvent & Solubility

In Vitro:

DMSO : ≥ 30 mg/mL (51.13 mM)

* "≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7042 mL	8.5209 mL	17.0419 mL
5 mM	0.3408 mL	1.7042 mL	3.4084 mL
10 mM	0.1704 mL	0.8521 mL	1.7042 mL

* Please refer to the solubility information to select the appropriate solvent.

References

[1]. Jo MJ, et al. Regulation of cancer cell death by a novel compound, C604, in a c-Myc-overexpressing cellular environment. Eur J Pharmacol. 2015 Dec 15;769:257-65.

[2]. Rivas-Carrillo JD, et al. Cell-permeable pentapeptide V5 inhibits apoptosis and enhances insulin secretion, allowing experimental single-donor islet transplantation in mice. Diabetes. 2007 May;56(5):1259-67. Epub 2007 Feb 7.

Cell Assay ^[1]	Cells (2.5×10⁴ cells/mL) are grown in 96-well plates in a final volume of 100 μL/well. After 24 h, cells are incubated with small molecules or vehicle (DMSO) for 48-96 h prior to harvest. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole (MTT) reagent (0.5 mg/mL), is added to each well during the final 2 h, and absorbance is measured. Cell growth is calculated as the ratio of absorbance obtained upon compound treatment to that obtained with vehicle (DMSO) treatment. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Jo MJ, et al. Regulation of cancer cell death by a novel compound, C604, in a c-Myc-overexpressing cellular environment. Eur J Pharmacol. 2015 Dec 15;769:257-65. [2]. Rivas-Carrillo JD, et al. Cell-permeable pentapeptide V5 inhibits apoptosis and enhances insulin secretion, allowing experimental single-donor islet transplantation in mice. Diabetes. 2007 May;56(5):1259-67. Epub 2007 Feb 7.

Cell Assay
^[1]

Cells (2.5×10⁴ cells/mL) are grown in 96-well plates in a final volume of 100 μL/well. After 24 h, cells are incubated with small molecules or vehicle (DMSO) for 48-96 h prior to harvest. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole (MTT) reagent (0.5 mg/mL), is added to each well during the final 2 h, and absorbance is measured. Cell growth is calculated as the ratio of absorbance obtained upon compound treatment to that obtained with vehicle (DMSO) treatment.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References