Epibrassinolide 纯度:>98.00% 10mg

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Epibrassinolide 纯度:>98.00% 10mg

市场价：: 1350.00

促销价：: ¥1350.00

货号：HY-N0848

品牌：MCE

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商品介绍
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Epibrassinolide 是一种天然油菜素类固醇(BR)衍生物，是一种植物调节剂，具有与哺乳动物类固醇相似的结构。Epibrassinolide在各种癌细胞中是一种潜在的凋亡诱导剂，而不影响非肿瘤细胞生长。

Description

Epibrassinolide is a natural brassinosteroid (BR) derivative, is a plant regulator with a similar structure to mammalian steroids. Epibrassinolide is a potential apoptotic inducer in various cancer cells without affecting the non-tumor cell growth.

IC₅₀ & Target

Plant growth regulator, Apoptosis inducer^[1]

In Vitro

Epibrassinolide (EBR) is a biologically active compound of the brassinosteroids, steroid-derived plant growth regulator family. Cells are incubated with various doses (0-100 μM) of Epibrassinolide for 24 or 48 h and cell viability is determined by MTT assay. Epibrassinolide induced cell viability loss in dose- and time-dependent manner compared to untreated samples in LNCaP and DU145 prostate cancer cells. Increasing concentrations of Epibrassinolide is more effective on LNCaP cell viability loss than DU145 cells suggesting that androgen-dependent cells are more sensitive to Epibrassinolide than androgen-independent prostate cancer cells. In further experiments, 25 μM Epibrassinolide is selected due to its moderate cytotoxic effect on both cell lines. The effect of Epibrassinolide treatment is examined on cell proliferation by counting the cell number within 96 h. A higher and earlier inhibition of cell proliferation is observed in LNCaP than DU145 cells^[1].

Solvent & Solubility

In Vitro:

DMSO : ≥ 5 mg/mL (10.40 mM)

* "≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0804 mL	10.4019 mL	20.8039 mL
5 mM	0.4161 mL	2.0804 mL	4.1608 mL
10 mM	0.2080 mL	1.0402 mL	2.0804 mL

* Please refer to the solubility information to select the appropriate solvent.

References

[1]. Obakan P, et al. Activation of polyamine catabolic enzymes involved in diverse responses against epibrassinolide-induced apoptosis in LNCaP and DU145 prostate cancer cell lines. Amino Acids. 2014 Mar;46(3):553-64.

Cell Assay ^[1]	LNCaP (CRL-1740) and DU145 (CRL-2698) human prostate cancer cell lines are used. PNT1a normal prostate epithelial cell line is used. Cells are grown in RPMI 1640 medium, supplemented with 10 % fetal bovine serum, 10,000 U penicillin/mL and 10 mg streptomycin/mL. Cells are cultured at 37°C in a humidified 5 % CO₂ incubator (HERA cell 150). Cells are seeded overnight and then treated with the desired drug concentrations. Cells are seeded at 1×10⁴ density per well in 96-well plates and exposed to various concentrations of Epibrassinolide for 24 and 48 h. 10 μL MTT dye (5 mg/mL) is added to each well and cells are kept at 37°C for 4 h. The resulting formazan crystals are solubilized in 200 μL DMSO. The density of the solubilized formazan is read at 570 nm spectrophotometrically^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Obakan P, et al. Activation of polyamine catabolic enzymes involved in diverse responses against epibrassinolide-induced apoptosis in LNCaP and DU145 prostate cancer cell lines. Amino Acids. 2014 Mar;46(3):553-64.

Cell Assay
^[1]

LNCaP (CRL-1740) and DU145 (CRL-2698) human prostate cancer cell lines are used. PNT1a normal prostate epithelial cell line is used. Cells are grown in RPMI 1640 medium, supplemented with 10 % fetal bovine serum, 10,000 U penicillin/mL and 10 mg streptomycin/mL. Cells are cultured at 37°C in a humidified 5 % CO₂ incubator (HERA cell 150). Cells are seeded overnight and then treated with the desired drug concentrations. Cells are seeded at 1×10⁴ density per well in 96-well plates and exposed to various concentrations of Epibrassinolide for 24 and 48 h. 10 μL MTT dye (5 mg/mL) is added to each well and cells are kept at 37°C for 4 h. The resulting formazan crystals are solubilized in 200 μL DMSO. The density of the solubilized formazan is read at 570 nm spectrophotometrically^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References