Cells are treated with serial dilutions of test molecules and incubated 4-6 days depending on cell line. Assessment of cellular growth and data reduction to generate IC50 values is done using Alamar Blue dye reduction assay^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Mice: When subcutaneous Karpas 299 tumor size reaches 300 mm³, three animals per group receives one injection of 10 mg antibody component/kg body weight of either cAC10-L1-MMAF₄ or cBR96-L1-MMAF₄ intravenously. Tumors are then removed and placed in optimal cutting temperature compound, and 5 μm-thin frozen tissue sections are stained using immunohistochemistry evaluation^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Doronina SO, et al. Enhanced activity of monomethylauristatin F through monoclonal antibody delivery: effects of linker technology on efficacy and toxicity. Bioconjug Chem. 2006 Jan-Feb;17(1):114-24.

768.42

C₃₉H₆₆ClN₅O₈

1415246-68-2

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Room temperature in continental US; may vary elsewhere