Flagelin 22 5mg

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Qiagen 凯杰 Biosharp Omega

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首页酶、辅酶及抑制剂MCE>

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市场价：: 3900.00

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货号：HY-P1568

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商品介绍
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Flagelin 22 是细菌鞭毛素的一个片段，在植物和藻类中都是有效的诱导剂。

Description

Flagelin 22, a fragment of bacterial flagellin, is an effective elicitor in both plants and algae.

In Vitro

Flagelin 22 (flg22) is a 22-amino-acid peptide, which corresponds to the highly conserved N-terminal region of flagellin, can induce immunity reaction in various plants such as tomato (Solanum lycopersicum), potato (Solanum tuberosum), tobacco (Nicotiana tabacum), and Arabidopsis thaliana. Flagelin 22 can induce oxidative bursts and hypersensitive responses (HR) in both female gametophytes and sporophytes of Saccharina japonica, indicating that algae and plants may share similar mechanisms for recognizing pathogens. After culturing the female gametophytes of S. japonica in the presence of Flagelin 22, flg15, flg14, and flg22D43A for 40 days, both Flagelin 22 and flg15 significantly induce growth inhibition of the algae at a concentration of 1 μM. The fresh weights of Flagelin 22- and flg15-challenged female gametophytes are less than one half of the control^[1].

Solvent & Solubility

In Vitro:

10 mM in H₂O

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.4400 mL	2.2002 mL	4.4005 mL
5 mM	0.0880 mL	0.4400 mL	0.8801 mL
10 mM	0.0440 mL	0.2200 mL	0.4400 mL

* Please refer to the solubility information to select the appropriate solvent.

References

[1]. Bojun Lu, et al. Defense responses in female gametophytes of Saccharina japonica (Phaeophyta) induced by flg22-derived peptides. Journal of Applied Phycology (2016), 28(3), 1793-1801.

Cell Assay ^[1]	One milliliter of the female gametophytes is added to 100 mL sterilized seawater containing 1 μM of Flagelin 22, flg15, flg14, and flg22D43A, respectively. Controls are sterilized seawater and 0.1% BSA in sterilized seawater. Gametophytes are grown at 10°C with a 24-h photoperiod at 50 μmol photons m^-2 s^-1. Sterilized seawater medium is provided with 0.2 mM KNO₃, 0.02mM KH₂PO₄, and 1 μM of the four respective peptides and refreshed every week. The gametophytes are briefly blotted dry, and the fresh weight is measured after 40 days^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Bojun Lu, et al. Defense responses in female gametophytes of Saccharina japonica (Phaeophyta) induced by flg22-derived peptides. Journal of Applied Phycology (2016), 28(3), 1793-1801.

Cell Assay
^[1]

One milliliter of the female gametophytes is added to 100 mL sterilized seawater containing 1 μM of Flagelin 22, flg15, flg14, and flg22D43A, respectively. Controls are sterilized seawater and 0.1% BSA in sterilized seawater. Gametophytes are grown at 10°C with a 24-h photoperiod at 50 μmol photons m^-2 s^-1. Sterilized seawater medium is provided with 0.2 mM KNO₃, 0.02mM KH₂PO₄, and 1 μM of the four respective peptides and refreshed every week. The gametophytes are briefly blotted dry, and the fresh weight is measured after 40 days^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Bojun Lu, et al. Defense responses in female gametophytes of Saccharina japonica (Phaeophyta) induced by flg22-derived peptides. Journal of Applied Phycology (2016), 28(3), 1793-1801.

Molecular Weight	2272.48
Formula	C₉₃H₁₆₂N₃₂O₃₄
CAS No.	304642-91-9
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Shipping	Room temperature in continental US; may vary elsewhere