Nifursol is a nitrofuran antibiotic which inhibits the growth of Histomonas meleagridis but is not lethal to the flagellated protozoan. Target: Antibacterial Nifursol can be analyzed and detected in tissues using intact 3,5-dinitrosalicylic acid hydrazide side chains along with electron-capture GC, spectrophotometric detection, and HPLC with ultraviolet light detection. In tissues, it has been observed that Nifursol is tightly bound to proteins and requires basic solutions such as ammonia to break the bonds between the two.