Nisin 10g

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Qiagen 凯杰 Biosharp Omega

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商品介绍
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Nisin 是由一组属于乳球菌和链球菌属的革兰氏阳性菌产生的乳酸链球菌肽。

Description

Nisin is a bacteriocin produced by a group of Gram-positive bacteria that belongs to Lactococcus and Streptococcus species.

In Vitro

Nisin is classified as a Type A (I) lantibiotic that is synthesized from mRNA and the translated peptide contains several unusual amino acids due to post-translational modifications. Nisin is an antimicrobial peptide produced by certain Gram-positive bacteria that include Lactococcus and Streptococcus species^[1].

Solvent & Solubility

In Vitro:

H₂O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH₄OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

References

[1]. Shin JM, et al. Biomedical applications of nisin. J Appl Microbiol. 2016 Jun;120(6):1449-65.

Molecular Weight	3354.07
Formula	C₁₄₃H₂₃₀N₄₂O₃₇S₇
CAS No.	1414-45-5
Storage	4°C, stored under nitrogen
Shipping	Room temperature in continental US; may vary elsewhere